Identification of Potential Biomarkers of Radiation Exposure in Blood Cells by Capillary Electrophoresis Time-of-Flight Mass Spectrometry
Identification of Potential Biomarkers of Radiation Exposure in Blood Cells by Capillary Electrophoresis Time-of-Flight Mass Spectrometry
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DOI：
10.3390/ijms21030812
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发表时间：
2020-02-01
影响因子：
5.6
通讯作者：
Moritake, Takashi
中科院分区：
文献类型：
--
作者：
Sun, Lue;Inaba, Yohei;Moritake, Takashi
生物剂量学是估算个人电离辐射暴露剂量的有用方法。研究发现，非细胞生物体液中的代谢物可用作生物剂量学中的标志物。血细胞中代谢物的水平可能会不同地反映健康状况或环境压力。在这里，我们报告了体内暴露于X射线后小鼠血细胞代谢物水平的变化。用毛细管电泳联用飞行时间质谱仪测定血细胞代谢物水平。暴露后，100种代谢物的水平发生了很大变化。我们确定了2-氨基丁酸、2‘-脱氧胞苷和胆碱作为辐射暴露的潜在有用标记物，并利用逐步回归建立了潜在的暴露剂量预测小组。血细胞代谢物水平可能是估算意外辐射事件中暴露剂量的有用生物标志物。
Biodosimetry is a useful method for estimating personal exposure doses to ionizing radiation. Studies have identified metabolites in non-cellular biofluids that can be used as markers in biodosimetry. Levels of metabolites in blood cells may reflect health status or environmental stresses differentially. Here, we report changes in the levels of murine blood cell metabolites following exposure to X-rays in vivo. Levels of blood cell metabolites were measured by capillary electrophoresis time-of-flight mass spectrometry. The levels of 100 metabolites were altered substantially following exposure. We identified 2-aminobutyric acid, 2'-deoxycytidine, and choline as potentially useful markers of radiation exposure and established a potential prediction panel of the exposure dose using stepwise regression. Levels of blood cell metabolites may be useful biomarkers in estimating exposure doses during unexpected radiation incidents.