Rheumatoid factors from patients with rheumatoid arthritis react with Des-Lys58-beta 2m, modified beta 2-microglobulin.
Rheumatoid factors from patients with rheumatoid arthritis react with Des-Lys58-beta 2m, modified beta 2-microglobulin.
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类风湿性关节炎患者的类风湿因子与 Des-Lys58-beta 2m（修饰的 β2-微球蛋白）发生反应。
DOI：
10.1111/j.1365-2249.1993.tb03414.x
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发表时间：
1993
影响因子：
4.6
通讯作者：
Malone,CC
中科院分区：
文献类型：
--
作者：
WilliamsJr,RC;Nissen,MH;Malone,CC
研究了从IgG柱亲和纯化的10种来自类风湿性关节炎患者的多克隆IgM类风湿因子（RF）制剂与天然β 2 m以及Lys 58-切割的β 2 m和Des-Lys 58-β 2 m（后者代表某些病理性人血清中存在的天然分子的切割产物）的ELISA反应性。大多数RF与天然形式的β 2 m呈阳性反应，但与裂解形式的β 2 m的反应性降低。通过与一系列浓度的Dcs-Lys 58-β 2 m预孵育抑制RF与天然β 2 m的结合，证明了溶液中裂解的β 2 m与RF之间的反应。相比之下，9种抗人β 2 m的鼠单克隆抗体中有8种显示出与天然β 2 m、Lys 58-切割的β 2 m和Des-Lys 58-β 2 m的近似等效结合。个体人RF与β 2 m的改变形式（Lys 58-切割的β 2 m和Des-Lys 58-β 2 m）之间的反应似乎与先前确定的β 2 m单氨基酸特异性平行，因为RF与赖氨酸58显示出强反应性，也显示出与缺少关键赖氨酸残基的Des-Lys 58-β 2 m的反应性显著降低。目前的研究表明，虽然人RF与Lys 58切割的β 2 m或Des-Lys 58-β 2 m反应，但观察到天然未改变的β 2 m具有优先反应性。
Ten polyclonal IgM rheumatoid factor (RF) preparations, affinity‐purified from IgG columns, from patients with rheumatoid arthritis were studied for their ELISA reactivity with native β2m in parallel with Lys58‐cleaved β2m and Des‐Lys58‐β2m, the latter representing cleavage products of the native molecule present in some pathologic human sera. Most RF showed positive reactions with the native form of β2m but reduced reactivity for the cleaved forms of β2m. Reactions between cleaved β2m and RF, in solution, were demonstrated by inhibition of RF binding to native β2m by prcincubation with a range of concentrations of Dcs‐Lys58‐β2m. By contrast, eight of nine murine MoAbs to human β2m showed approximately equivalent binding to native β2m, Lys58‐cleaved β2m, and Des‐Lys58‐β2m. Reactions between individual human RF and the altered forms of β2m (Lys58‐cleaved β2m and Des‐Lys58‐β2m) appeared to parallel the previously determined β2m single ammo acid specificities, in that RF’ showing strong reactivity with Lysine 58 also showed a significant diminished reactivity with the Des‐Lys58‐β2m lacking the critical lysine residue. The present studies demonstrate that while human RF react with Lys58‐cleaved β2m or Des‐Lys58‐β2m, preferential reactivity is observed for native unaltered β2m.