Super-Resolution Imaging Approaches for Quantifying F-Actin in Immune Cells.
Super-Resolution Imaging Approaches for Quantifying F-Actin in Immune Cells.
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DOI：
10.3389/fcell.2021.676066
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发表时间：
2021
影响因子：
5.5
通讯作者：
Owen DM
中科院分区：
文献类型：
--
作者：
Garlick E;Thomas SG;Owen DM
免疫细胞包括一组不同的细胞，它们经历一系列复杂的生物过程，这些过程必须受到严格的调控。实现这一点的细胞机器的一个关键组成部分是细胞骨架。因此，对细胞骨架的结构和动力学进行成像和定量描述是一个重要的研究目标。光学显微镜非常适合这项任务。在这里，我们回顾了最新的国家的最先进的方法标记的细胞骨架，荧光显微镜硬件适合这种成像和定量统计分析软件适用于描述细胞骨架结构。我们还强调了当前的挑战和未来发展的领域。
Immune cells comprise a diverse set of cells that undergo a complex array of biological processes that must be tightly regulated. A key component of cellular machinery that achieves this is the cytoskeleton. Therefore, imaging and quantitatively describing the architecture and dynamics of the cytoskeleton is an important research goal. Optical microscopy is well suited to this task. Here, we review the latest in the state-of-the-art methodology for labeling the cytoskeleton, fluorescence microscopy hardware suitable for such imaging and quantitative statistical analysis software applicable to describing cytoskeletal structures. We also highlight ongoing challenges and areas for future development.