A role for matrix metalloproteinases and tumor host interaction in hepatocellular carcinomas
A role for matrix metalloproteinases and tumor host interaction in hepatocellular carcinomas
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DOI：
10.1016/s0002-9610(02)00833-4
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发表时间：
2002-05-01
影响因子：
3
通讯作者：
Chung, SW
中科院分区：
文献类型：
--
作者：
McKenna, GJ;Chen, YL;Chung, SW
背景：肝细胞癌（HCC）发生在肝脏损伤重塑，由基质金属蛋白酶（MMP）完成。转移涉及也由MMP活性引起的基底膜侵袭。MMP及其内源性抑制因子（TIMP）表达的改变可能是HCC转移的因素。方法：将HCC标本和淋巴结（n = 7）以及来自器官供体的正常淋巴组织（n = 8）在液氮中速冻并沉淀mRNA。用MMP（MMP 2、MMP 7、MMP 9）和TIMP（TIMP 1、TIMP 2）引物进行一系列逆转录聚合酶链反应（RT-PCR）。结果：MMP 2：TIMP 2 mRNA在正常组织和肿瘤边缘组织中的表达比例均高于肿瘤组织。结论：胃癌引流淋巴结中MMP 2：TIMP 2：mRNA表达均发生改变，MMP 2：TIMP 2：mRNA表达均发生改变，MMP 2：TIMP 2：mRNA表达均发生改变。这可能反映了调节肿瘤转移的宿主-肿瘤相互作用。(C)2002年，Excerpta Medica，Inc. All rights reserved.
Background: Hepatocellular carcinoma (HCC) occur in livers with injury-remodeling, accomplished by enzymes called matrix metalloproteinases (MMP). Metastasis involves basement membrane invasion also caused by MMP activity. Alterations in MMP expression and their endogenous inhibitor (TIMP) may factor in HCC metastasis.Methods: HCC specimens and lymph nodes (n = 7), and normal lymph tissue from organ donors (n = 8), were snap-frozen in liquid nitrogen and the mRNA precipitated. A series of reverse transcription-polymerase chain reactions (RT-PCR) were performed using MMP (MMP2, MMP7, MMP9) primers and TIMP (TIMP1, TIMP2) primers. These were semiquantitatively analyzed by comparing concentration with constitutive GADPH expression.Results: There is an increase in MMP2:TIMP2 mRNA expression ratio in the normal and tumor margin tissue compared to the tumor. There are increases in all MMP and TIMP mRNA expression (except TIMP1) and alterations in ail of the MMP:TIMP expression ratios in the draining lymph node.Conclusions: Alterations exist in MMP2:TIMP2: expression at the margin, and all of the MMPs in the draining lymph nodes. This likely reflects a host-tumor interaction that regulates tumor metastasis. (C) 2002 Excerpta Medica, Inc. All rights reserved.