Clinical perspectives in integrating whole-genome sequencing into the investigation of healthcare and public health outbreaks - hype or help?
Clinical perspectives in integrating whole-genome sequencing into the investigation of healthcare and public health outbreaks - hype or help?
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DOI：
10.1016/j.jhin.2020.11.001
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发表时间：
2021-03
期刊：
影响因子：
--
通讯作者：
Holden MTG
中科院分区：
文献类型：
--
作者：
Parcell BJ;Gillespie SH;Pettigrew KA;Holden MTG
疫情对患者安全构成重大风险，调查成本高昂且耗时。有针对性的感染预防和控制措施的实施依赖于感染预防和控制小组能够获得准确检测耐药性的快速结果，以及能够提供有关分离株相关性的临床有用信息的分型结果。目前，确定是否发生了传播可能是一项重大挑战。传统的分型结果并不总是具有足够的粒度或稳健性来明确定义菌株，并且并不总是有足够的流行病学数据来建立患者与环境之间的联系。全基因组测序（WGS）已成为最终的基因分型工具，但尚未完全跨越研究方法与常规临床诊断微生物学技术之间的鸿沟。临床WGS服务于2014年正式成立，作为苏格兰医疗保健相关感染预防研究所的一部分，以确认或反驳苏格兰各地医院环境中的疫情。本文描述了作者的经验，目的是为使用WGS调查医疗保健和公共卫生暴发的实际应用提供新的见解。解决方案，以克服障碍，实施这项技术在临床环境中提出。
Outbreaks pose a significant risk to patient safety as well as being costly and time consuming to investigate. The implementation of targeted infection prevention and control measures relies on infection prevention and control teams having access to rapid results that detect resistance accurately, and typing results that give clinically useful information on the relatedness of isolates. At present, determining whether transmission has occurred can be a major challenge. Conventional typing results do not always have sufficient granularity or robustness to define strains unequivocally, and sufficient epidemiological data are not always available to establish links between patients and the environment. Whole-genome sequencing (WGS) has emerged as the ultimate genotyping tool, but has not yet fully crossed the divide between research method and routine clinical diagnostic microbiological technique. A clinical WGS service was officially established in 2014 as part of the Scottish Healthcare Associated Infection Prevention Institute to confirm or refute outbreaks in hospital settings from across Scotland. This article describes the authors' experiences with the aim of providing new insights into practical application of the use of WGS to investigate healthcare and public health outbreaks. Solutions to overcome barriers to implementation of this technology in a clinical environment are proposed.
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DOI：
10.1007/s10096-017-3024-6
发表时间：
2017-11
期刊：
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
影响因子：
--
作者：
Tagini F;Greub G
通讯作者：
Greub G
影响因子：
5.2
作者：
Ibrahim, George M.;Morin, Paul M.
通讯作者：
Morin, Paul M.
影响因子：
6.9
作者：
Parcell, B. J.;Oravcova, K.;Gillespie, S. H.
通讯作者：
Gillespie, S. H.
影响因子：
3
作者：
Fox, Edward M.;deLappe, Niall;Jordan, Kieran
通讯作者：
Jordan, Kieran
影响因子：
3.7
作者：
Mellmann A;Harmsen D;Cummings CA;Zentz EB;Leopold SR;Rico A;Prior K;Szczepanowski R;Ji Y;Zhang W;McLaughlin SF;Henkhaus JK;Leopold B;Bielaszewska M;Prager R;Brzoska PM;Moore RL;Guenther S;Rothberg JM;Karch H
通讯作者：
Karch H