Measuring the Replicative Lifespan of Saccharomyces cerevisiae Using the HYAA Microfluidic Platform.
Measuring the Replicative Lifespan of Saccharomyces cerevisiae Using the HYAA Microfluidic Platform.
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DOI：
10.1007/978-1-0716-0592-9_1
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Dang W
中科院分区：
文献类型：
--
作者：
Yu R;Jo MC;Dang W
芽殖酵母（Saccharomyces cerevisiae）的复制老化已经成为剖析老化过程分子机制的有用模型。传统上，复制寿命（RLS）是通过人工从子细胞中分离母细胞来测量的，这是一个非常繁琐的过程。自2012年以来，已经开发了几种微流体系统来自动化解剖过程，大大加快了RLS的测定。在这里，我们描述了使用基于HYAA芯片设计的市售微流体系统进行RLS测量的详细方案，该系统可以在单个实验中收集多达8,000个细胞的数据。
The replicative aging of the budding yeast, Saccharomyces cerevisiae, has been a useful model for dissecting the molecular mechanisms of the aging process. Traditionally, the replicative lifespan (RLS) is measured by manually dissecting mother cells from daughter cells, which is a very tedious process. Since 2012, several microfluidic systems have been developed to automate the dissection process, significantly accelerating RLS determination. Here, we describe a detailed protocol of RLS measurement using a commercially available microfluidic system based on the HYAA chip design, which enables data collection of up to 8,000 cells in a single experiment.