Effects of dietary zinc oxide and selenium nanoparticles on growth performance, immune responses and enzyme activity in rohu, Labeo rohita (Hamilton)
Effects of dietary zinc oxide and selenium nanoparticles on growth performance, immune responses and enzyme activity in rohu, Labeo rohita (Hamilton)
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DOI：
10.1111/anu.12874
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发表时间：
2018-12
影响因子：
3.5
通讯作者：
P. Swain;R. Das;Aakankshya Das;S. K. Padhi;K. Das;S. Mishra
中科院分区：
文献类型：
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作者：
P. Swain;R. Das;Aakankshya Das;S. K. Padhi;K. Das;S. Mishra
本试验旨在评价饲粮中添加氧化锌（ZnO）和硒（Se）纳米颗粒（NPs）对罗氏罗虎生长、免疫和酶谱的影响。在整个试验期间，在基础饲料中添加实验室合成的纳米颗粒，即氧化锌纳米颗粒（10 mg/kg）和硒纳米颗粒（0.3 mg/kg）。每隔30天，随机收集处理鱼和对照鱼，进行生长、非特异性免疫参数和血清酶测定。观察到，与对照组（仅基础饲料）相比，生长和非特异性免疫参数如呼吸爆发、溶菌酶和髓过氧化物酶活性显著（p< 0.05）增加。与对照组相比，治疗组血清酶谱如乳酸脱氢酶和碱性磷酸酶活性下调（p< 0.05），治疗组超氧化物歧化酶和乙酰胆碱酯酶活性上调。毒株嗜水气单胞菌攻毒后，处理组的相对存活率（RPS）为60.00±8.82%，显著高于对照组（45.00±6.17%）。上述结果提示，饲粮中添加氧化锌和硒纳米颗粒可刺激仔猪免疫力，增强仔猪对细菌感染的抵抗力。rohita。
A 120‐day feeding trial was conducted to assess the effects of dietary supplementation of zinc oxide (ZnO) and selenium (Se) nanoparticles (NPs) on growth, immunological and enzymatic profiles in rohu,Labeo rohita. Fishes were fed with basal diet incorporated with laboratory‐synthesized nanoparticles, viz., zinc oxide nanoparticles—10 mg/kg and Se nanoparticles—0.3 mg/kg throughout the experimental period. At an interval of every 30 days, treated and control fishes were randomly collected and subjected to estimation of growth, non‐specific immune parameters and serum enzyme assays. It was observed that there was significant (p< 0.05) increase in growth and non‐specific immune parameters like respiratory burst, lysozyme and myeloperoxidase activities as compared to the control group (only basal diet). Serum enzymatic profiles such as lactate dehydrogenase and alkaline phosphatase activities down‐regulated (p< 0.05) in treated group compared to control group, and superoxide dismutase and acetylcholine esterase activity up‐regulated in treated group. The relative percentage survival (RPS) found to be significantly higher in treated groups (60.00 ± 8.82%) as compared to control group (45.00 ± 6.17%) while challenged with virulent bacterial strainAeromonas hydrophila. These results indicated that dietary supplementation of zinc oxide (ZnO) and selenium (Se) nanoparticles stimulates immunity and enhances resistance to bacterial infection inL. rohita.