Spectrin subtypes in mammalian brain.
Spectrin subtypes in mammalian brain.
复制标题
哺乳动物大脑中的血影蛋白亚型。
DOI：
10.1002/bies.950050108
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Zagon,IS
中科院分区：
文献类型：
--
作者：
Goodman,SR;Riederer,BM;Zagon,IS
哺乳动物神经细胞含有至少两种形式的脑血影蛋白：主要位于神经元的轴突和突触前末梢中的脑血影蛋白（240/235），以及在神经元的细胞体、树突和突触后末梢中发现的脑血影蛋白（240/235 E）。脑血影蛋白（240/235 E）也存在于某些神经胶质细胞类型中。针对红细胞血影蛋白的抗体仅检测脑血影蛋白（240/235 E），而针对从轴突和突触膜分离的脑血影蛋白的抗体检测脑血影蛋白（240/235）。先前关于脑血影蛋白在光学显微镜水平定位的文献中的明显差异无疑是由于不同的实验室检测不同的脑血影蛋白亚型，基于用于免疫组织化学的特定抗体。在这篇综述中，我们（1）讨论了支持哺乳动物脑血影蛋白至少存在两种不同亚型的数据，（2）解释了这些结果如何调和先前关于血影蛋白在神经细胞内定位的差异，（3）提出了这些发现的未来意义。
Mammalian neural cells contain at least two forms of brain spectrin: brain spectrin (240/235) which is located primarily in the axons and presynaptic terminals of neurons, and brain spectrin (240/235E) which is found in the cell bodies, dendrites and postsynaptic terminals of neurones. Brain spectrin (240/235E) is also found in certain glial cell types. Antibodies against red blood cell spectrin detect only brain spectrin (240/235E), while antibodies against brain spectrin isolated from axonal and synaptic membranes detect brain spectrin (240/235). Previous apparent discrepancies in the literature concerning brain spectrin localization at the light microscope level were undoubtedly due to different laboratories detecting distinct brain spectrin subtypes, based on the particular antibody being utilized for immunohistochemistry. In this review we (1) discuss the data supporting the presence of at least two distinct subtypes of mammalian brain spectrin, (2) explain how these results reconcile previous discrepancies concerning the localization of spectrin within neural cells, and (3) suggest the future implications of these findings.