DISTRIBUTION OF VITRONECTIN IN PLASMA AND LIVER-TISSUE - RELATIONSHIP TO CHRONIC LIVER-DISEASE
DISTRIBUTION OF VITRONECTIN IN PLASMA AND LIVER-TISSUE - RELATIONSHIP TO CHRONIC LIVER-DISEASE
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DOI：
10.1002/hep.1840200606
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发表时间：
1994-12-01
期刊：
影响因子：
13.5
通讯作者：
KAWASAKI, H
中科院分区：
文献类型：
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作者：
KOBAYASHI, J;YAMADA, S;KAWASAKI, H
为了阐明玻璃体粘连蛋白的临床意义，我们将慢性肝病患者血浆玻璃体粘连蛋白浓度与血清纤维标志物和肝功能检查值进行比较。我们还采用酶联免疫吸附法和酶免疫组化法测定了玻璃体粘连素在肝组织中的定位。慢性肝病患者血浆玻璃体粘连蛋白浓度明显低于健康对照组，与肝病的严重程度有关。血浆玻璃体粘连蛋白水平与纤维标志物无相关性，但与血清白蛋白和凝血酶原时间有显著相关性。另一方面，慢性肝病患者肝组织中玻璃体粘连蛋白的含量明显高于正常对照组。光镜下正常肝脏门静脉区可见玻璃体粘连蛋白。在慢性肝炎和肝硬化患者中，玻璃体粘连蛋白见于门静脉和中央静脉周围的结缔组织以及局部和局灶性坏死区域。这些结果表明，玻璃体连接蛋白通过修复和纤维化过程沉积在损伤组织中，并作为粘附蛋白发挥重要作用。此外，慢性肝病患者血浆玻璃体粘连蛋白水平较低可能是由于其在损伤组织中的合成或沉积减少或两者兼而有之。
To clarify the clinical significance of vitronectin, we compared the concentration of plasma vitronectin with serum fibrous markers and liver function test values in patients with chronic liver diseases. We also evaluated the vitronectin content in the liver by means of enzyme-linked immunosorbent assay and the localization of vitronectin in liver tissue with enzyme immunohistochemistry. In chronic liver disease, the concentration of plasma vitronectin was significantly lower than that in healthy controls, being related to the severity of liver disease. The plasma levels of vitronectin showed no correlation to fibrous markers but a significant correlation with those of serum albumin and prothrombin time. On the other hand, the content of vitronectin in liver tissue was significantly increased in chronic liver disease compared with that in normal controls. In the normal liver, vitronectin was observed in the portal area by light microscopy. In chronic hepatitis and cirrhosis, vitronectin was found in the connective tissue around the portal and central veins and in the areas of piecemeal and focal necrosis. These findings suggested that vitronectin is deposited in injured tissue through the process of repair and fibrosis and plays an important role as an adhesive protein. Moreover, the lower levels of plasma vitronectin in chronic liver disease may be due to its decreased synthesis, deposition or both in injured tissue.