Creating a synthetic lichen: Mutualistic co-culture of fungi and extracellular polysaccharide-secreting cyanobacterium Nostoc PCC 7413
Creating a synthetic lichen: Mutualistic co-culture of fungi and extracellular polysaccharide-secreting cyanobacterium Nostoc PCC 7413
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DOI：
10.1016/j.algal.2019.101755
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发表时间：
2020-01-01
影响因子：
5.1
通讯作者：
Betenbaugh, Michael J.
中科院分区：
文献类型：
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作者：
Li, Tingting;Jiang, Liqun;Betenbaugh, Michael J.
为了产生合成地衣，将分泌胞外多糖（EPS）的蓝细菌Nostoc PCC 7413与真菌构巢曲霉（Aspergillus nidulans）和黑曲霉（Aspergillus尼日尔）物种一起培养，而没有外来有机碳。蓝细菌上清和收获的EPS都支持真菌生长，而真菌上清略微促进念珠藻生长。此外，当念珠藻和A.在pH 6缓冲BG-11培养基中，总生物量比纯念珠藻培养物高约3倍。在共培养中产生的脂肪酸的光谱不同于那些个别的蓝藻和真菌物种。与单培养物相比，C18：0和C18：1的脂肪酸级分在共培养物中减少或居中，而C16：1和C18：3脂肪酸级分在共培养物中增加，表明共培养后脂肪酸生物合成的转变。我们的研究建立了一个低成本的互利共生共培养平台，由蓝藻和丝状真菌生产生物质和生物燃料前体具有潜在的商业应用。
In order to create synthetic lichens, extracellular polysaccharide (EPS)-secreting cyanobacterium Nostoc PCC 7413 was cultured together with fungal Aspergillus nidulans and Aspergillus niger species without extraneous organic carbon. Cyanobacterial supernatants and harvested EPS both supported fungal growth, while fungal supernatants slightly enhanced Nostoc growth. Furthermore, when Nostoc and A. nidulans were co-cultured together, total biomass was approximately 3-fold higher than the axenic Nostoc cultures in pH 6 buffered BG-11 media. The spectrum of fatty acids generated in co-culture differed from those of the individual cyanobacterial and fungal species. The fatty acid fractions of C18:0 and C18:1 were reduced or intermediate in co-cultures compared to mono-cultures while fractions of C16:1 and C18:3 fatty acids increased in co-culture, suggesting a shift in the fatty acid biosynthesis following co-cultivation. Our study establishes a low-cost mutualistic co-culture platform composed of cyanobacteria and filamentous fungi for producing biomass and biofuel precursors with potential commercial applications.