Sample Preconcentration Protocols in Microfluidic Electrophoresis.

Sample Preconcentration Protocols in Microfluidic Electrophoresis.
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微流体电泳中的样品预浓缩方案。

DOI:
10.1007/978-1-4939-8964-5_4
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发表时间:
2018
影响因子:
--
通讯作者:
K. Otsuka
K. Otsuka
中科院分区:
--
文献类型:
--
作者:
F. Kitagawa;K. Otsuka

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微流控通道中的电泳在线样品预富集技术提高了分离前的灵敏度。在各种技术中,最重要的场放大样品堆积和扫描的交叉通道微芯片上进行了演示。作为一种新的微流控预富集方法,本文还提出了一种在直通道芯片上使用电渗流泵(LVSEP)进行大体积样品堆积的方法,该方法可以省去复杂的样品进样电压程序。在本章中,我们描述了如何准备以及如何在微芯片电泳中运行这些在线样品预浓缩方法。
Electrophoretic on-line sample preconcentration techniques in microfluidic channels improve the sensitivity prior to the separation. Among various techniques, the most important field-amplified sample stacking and sweeping on cross-channel microchips are demonstrated. As a novel microfluidic preconcentration approach, a large-volume sample stacking with electroosmotic flow pump (LVSEP) on straight-channel chips is also presented, which can omit a complicated voltage program for sample injection processes. In this chapter, we describe how to prepare and how to run these on-line sample preconcentration methods in microchip electrophoresis.
DOI: --
发表时间: 2010
期刊:
影响因子: --
作者:
Takayuki Kawai;Kenji Sueyoshi;Fumihiko Kitagawa;Koji Otsuka
通讯作者: Koji Otsuka