Alterations in lipidome and metabolome profiles of Nannochloropsis salina in response to reduced culture temperature during sinusoidal temperature and light
Alterations in lipidome and metabolome profiles of Nannochloropsis salina in response to reduced culture temperature during sinusoidal temperature and light
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DOI：
10.1016/j.algal.2018.03.001
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发表时间：
2018-06
期刊：
影响因子：
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通讯作者：
Stephanie M. Willette;Saba S Gill;Barry N. Dungan;Tanner M. Schaub;Jacqueline M. Jarvis;R. Hilaire;F. Holguin
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文献类型：
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作者：
Stephanie M. Willette;Saba S Gill;Barry N. Dungan;Tanner M. Schaub;Jacqueline M. Jarvis;R. Hilaire;F. Holguin
研究了盐生微绿球藻对四种次适培养温度的代谢反应，重点研究了脂肪酸和脂肪的代谢。培养物在受控环境光生物反应器中培养。在3种低温胁迫(5 °C、10 °C、15 °C)下，以生长速率和光合作用产量为特征的生理表现均下降。然而，在20 °C下培养的生物量生产力和总体生长与在最适温度(25 °C)下培养的相似。脂肪酸生产率在所有亚最适温度下都得到了提高，总脂肪酸和二十碳五烯酸的含量在20 °C时都静态地升高。脂肪的积累只在冷胁迫下观察到；然而，在所有温度变量中都发生了脂肪周转和多不饱和脂肪酸的富集化。此外，在极性和中性脂质池中都发生了广泛的脂质重塑，特别是在单半乳糖二酰甘油和三酰甘油池中。虽然20 °C是二十碳五烯酸和总脂肪酸生产率的最佳温度，但冷胁迫是必要的，以诱导三酰甘油库中生产力和多不饱和脂肪酸的浓缩。
Metabolic response to four suboptimal cultivation temperatures was explored inNannochloropsis salina, with an emphasis on fatty acid and lipid metabolism. Cultures were cultivated in controlled environmental photobioreactors. Physiological performance, characterized by growth rate and photosynthetic output, were reduced at all three cold stress temperatures (5 °C, 10 °C, 15 °C). However, biomass productivity and overall growth for cultures at 20 °C was like that of those grown at the optimal temperature (25 °C). Fatty acid productivity was elevated at all suboptimal temperatures, and both total fatty acid and eicosapentaenoic acid content were statically elevated at 20 °C. Lipid accumulation was observed exclusively in response to cold stress; however, lipid turnover and enrichment in polyunsaturated fatty acids occurred in all temperature variants. In addition, extensive lipid remodeling occurred in both polar and neutral lipid pools, specifically in monogalactosyl diacylglycerol and triacylglycerol pools. While 20 °C is optimal for eicosapentaenoic acid and total fatty acid productivity, cold stress is necessary to induce both productivity and polyunsaturated fatty acid enrichment in triacylglycerol pools.