Molecular detection and identification of Babesia bovis and Babesia bigemina in cattle in northern Thailand
Molecular detection and identification of Babesia bovis and Babesia bigemina in cattle in northern Thailand
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DOI：
10.1007/s00436-012-2960-4
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发表时间：
2012-09-01
影响因子：
2
通讯作者：
Xuan, Xuenan
中科院分区：
文献类型：
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作者：
Cao, Shinuo;Aboge, Gabriel Oluga;Xuan, Xuenan
虽然感染牛巴贝斯虫和二重巴贝斯虫给泰国北部养牛业造成了经济损失，但该地区目前存在的巴贝斯虫分离株信息不足。因此，为了确定巴贝斯虫分离株之间的流行率和遗传关系，我们筛选了来自泰国北部清莱省、清迈省和伦邦省的200头牛的血液样本。针对牛芽胞杆菌球体蛋白2(BboSBP2)和双链杆菌棒状杆菌相关蛋白1a(BbiRAP-1a)基因的套式聚合酶链式反应显示，牛和双链杆菌的感染率分别为12%和21%，而混合感染的样本为6.5%。清莱、清迈和伦邦的牛黄杆菌感染率分别为9.5%、3.7%和25.5%。双链球菌在清莱、清迈和伦邦的患病率分别为15.8%、12.9%和39.2%。牛分枝杆菌和双链球菌混合感染清莱为6.3%，清迈为1.9%，伦邦为13.7%。BboSBP2基因或BbiRAP-1a基因在泰国北部三省的巴贝斯虫分离株之间具有相同的序列。进一步利用内部转录间隔区基因进行分析，发现泰国北部至少有4种牛型芽胞杆菌和5种双链杆菌，但不同分离株的序列存在很大的遗传差异。总体而言，我们已经证明了巴贝斯虫在泰国北部的高流行率和多型性，需要设计有效的牛巴贝斯虫病控制方案。
Although Babesia bovis and Babesia bigemina infections cause economic losses in the cattle industry in northern Thailand, there is inadequate information on Babesia isolates present in the area. Therefore, to determine the prevalence and genetic relationship between Babesia isolates, we screened 200 blood samples of cattle from Chiang Rai, Chiang Mai, and Lumpang provinces of northern Thailand. A nested polymerase chain reaction using primers targeting B. bovis spherical body protein 2 (BboSBP2) and B. bigemina rhoptry-associated protein 1a (BbiRAP-1a) genes revealed a prevalence of 12 and 21 % for B. bovis and B. bigemina, respectively, while that of mixed infections was 6.5 % samples. The prevalences of B. bovis in Chiang Rai, Chiang Mai, and Lumpang were 9.5, 3.7, and 25.5 %, respectively. For B. bigemina, the prevalences were 15.8, 12.9, and 39.2 % in Chiang Rai, Chiang Mai, and Lumpang, respectively. Mixed infections with B. bovis and B. bigemina were 6.3 % in Chiang Rai, 1.9 % in Chiang Mai, and 13.7 % in Lumpang. The identical sequences of either BboSBP2 gene or BbiRAP-1a gene were shared among the Babesia isolates in the three provinces of northern Thailand. Further analysis using the internal transcribed spacer gene revealed at least four genotypes for B. bovis and five genotypes for B. bigemina in northern Thailand, while the sequences present great genetic diversities in the different isolates. Overall, we have demonstrated a high prevalence and polymorphism of Babesia parasites in northern Thailand calling for the need to design effective control programs for bovine babesiosis.