The anti-allergic activity of the acetate fraction of Schinus terebinthifolius leaves in IgE induced mice paw edema and pleurisy
The anti-allergic activity of the acetate fraction of Schinus terebinthifolius leaves in IgE induced mice paw edema and pleurisy
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DOI：
10.1016/j.intimp.2008.06.012
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发表时间：
2008-11-01
影响因子：
5.6
通讯作者：
Mueller de Oliveira Henriques, Maria das Gracas
中科院分区：
文献类型：
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作者：
Cavalher-Machado, Simone Campos;Rosas, Elaine Cruz;Mueller de Oliveira Henriques, Maria das Gracas
Schinus是漆树科的一个属，包含广泛用于民间医药的巴西胡椒树Schinus terebinthifolius。我们研究了S. terebinthifolius Raddi（ST组分）。HPLC分析表明，没食子酸、没食子酸甲酯和1，2，3，4，6-五没食子酰葡萄糖是该馏分的主要芳香成分。用ST组分（100 mg/kg）口服预处理显著抑制由化合物48/80（100 ng/爪）诱导的爪水肿，并在较小程度上抑制过敏性爪水肿（OVA，3 μ g/爪）。ST组分（100和200 mg/kg）还抑制组胺（100 μ g/爪）诱导的水肿，防止肥大细胞脱颗粒，从而防止C 48/80（5 μ g/mL）诱导的Wistar大鼠腹膜肥大细胞中组胺释放。在用没食子酸甲酯和1，2，3，4，6-五没食子酰葡萄糖（100 μ g/mL）（从乙酸乙酯级分分离的化合物）预处理肥大细胞后也观察到这种组胺抑制。ST组分（100 mg/kg）的预处理显著抑制胸腔内注射OVA（12.5 μ g/腔）后24 h胸膜腔中总白细胞和嗜酸性粒细胞的蓄积。这种作用与抑制胸腔灌洗液中的CCL 11/eotaxin和CCL 5/RANTES有关。用该级分（100 mg/kg）预处理未能减少LPS注射到胸膜腔（250 ng/腔）后观察到的细胞流入。这些发现证明了ST组分的抗过敏作用，包括抑制肥大细胞脱粒和嗜酸性粒细胞流入胸膜腔引起的水肿形成和组胺释放，这可能反映在回收的胸膜灌洗液中趋化因子水平降低。(C)2008 Elsevier B. V.保留所有权利。
Schinus is a genus of the Anacardiaceae family and contains Schinus terebinthifolius, the Brazilian pepper tree that is widely used in folk medicine. We investigate the anti-allergic activity of the ethyl acetate fraction of S. terebinthifolius Raddi (ST fraction). HPLC analysis reveled that gallic acid, methyl gallate and 1,2,3,4,6-pentagalloylglucose are the major aromatic components of the fraction. Oral pre-treatment with the ST fraction (100 mg/kg) significantly inhibited paw edema induced by compound 48/80 (100 ng/paw) and to a lesser extent, the allergic paw edema (OVA, 3 mu g/paw). The ST fraction (100 and 200 mg/kg) also inhibited the edema induced by histamine (100 mu g/paw), preventing mast cell degranulation and, consequently, histamine release in Wistar rat peritoneal mast cells induced by C 48/80 (5 mu g/mL). This histamine inhibition was also observed after mast cell pre-treatment with both methyl gallate and 1,2,3,4,6-pentagalloylglucose (100 mu g/mL), the isolated compounds from the ethyl acetate fraction. Pre-treatment with the ST fraction (100 mg/kg) significantly inhibited total leukocyte and eosinophil accumulation in pleural cavities 24 h after the intrathoracic injection of OVA (12.5 mu g/cavity). This effect was related to the inhibition of CCL11/eotaxin and CCL5/RANTES in pleural lavage fluid. Pre-treatment with this fraction (100 mg/kg) failed to reduce the cell influx that was observed after LPS-injection into pleural cavity (250 ng/cavity). These findings demonstrate the anti-allergic effect of the ST fraction, which includes the inhibition of edema formation and histamine release caused by mast cell degranulation and eosinophil influx into the pleural cavity probably reflected by the decreased levels of chemokines in recovered pleural lavage fluid. (C) 2008 Elsevier B.V. All rights reserved.