Experimental-computational evaluation of human bone marrow stromal cell spreading on trabecular bone structures.
Experimental-computational evaluation of human bone marrow stromal cell spreading on trabecular bone structures.
复制标题
人骨髓基质细胞在小梁骨结构上扩散的实验计算评估。
DOI：
10.1007/s10439-009-9676-3
复制
发表时间：
2009
影响因子：
3.8
通讯作者：
Sengers BG
中科院分区：
文献类型：
--
作者：
Sengers BG
细胞定植不足的问题严重影响了大孔骨再生支架的临床应用。鉴于用于组织工程的各种不同的支架结构，导出与支架结构无关的细胞定殖的关系是必要的。为了研究与营养限制无关的3D结构上的细胞群扩散，开发了一种体外培养系统，该系统由接种人骨髓基质细胞的人小梁骨薄片组成，结合专用μCT成像和细胞群扩散的计算建模。只有第一阶段ofin vitroscaffold殖民地解决，其中细胞迁移和增殖的阶段时，骨表面被覆盖作为一个单层，进一步的组织形成的关键先决条件。结果证实了该模型的能力，以代表实验观察到的细胞群体扩散。计算模型的主要优点是，通过结合复杂的3D结构，可以根据内在迁移参数定量表征细胞行为，这可能用于预测不同的大孔支架，但需要额外的实验验证。这种类型的建模将被证明是有用的预测细胞定植和改善骨骼组织工程的策略。
The clinical application of macro-porous scaffolds for bone regeneration is significantly affected by the problem of insufficient cell colonization. Given the wide variety of different scaffold structures used for tissue engineering it is essential to derive relationships for cell colonization independent of scaffold architecture. To study cell population spreading on 3D structures decoupled from nutrient limitations, anin vitroculture system was developed consisting of thin slices of human trabecular bone seeded with Human Bone Marrow Stromal Cells, combined with dedicated μCT imaging and computational modeling of cell population spreading. Only the first phase ofin vitroscaffold colonization was addressed, in which cells migrate and proliferate up to the stage when the surface of the bone is covered as a monolayer, a critical prerequisite for further tissue formation. The results confirm the model’s ability to represent experimentally observed cell population spreading. The key advantage of the computational model was that by incorporating complex 3D structure, cell behavior can be characterized quantitatively in terms of intrinsic migration parameters, which could potentially be used for predictions on different macro-porous scaffolds subject to additional experimental validation. This type of modeling will prove useful in predicting cell colonization and improving strategies for skeletal tissue engineering.