ORIGIN AND INHERITANCE OF DWARFING BY THE CITRUS ROOTSTOCK PONCIRUS-TRIFOLIATA FLYING-DRAGON
ORIGIN AND INHERITANCE OF DWARFING BY THE CITRUS ROOTSTOCK PONCIRUS-TRIFOLIATA FLYING-DRAGON
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DOI：
10.21273/jashs.120.2.286
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发表时间：
1995-03-01
影响因子：
1.9
通讯作者：
ROOSE, ML
中科院分区：
文献类型：
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作者：
CHENG, FSH;ROOSE, ML
‘飞龙’PonCirus trifoliata L.Raf.是柑橘的矮化砧木。对‘飞龙’自由授粉苗木群体的矮化力遗传进行了研究。分子标记基因分型表明，所有苗木均来源于自交。以‘卡特瓦伦西亚’橙为试材，对其子代幼苗进行发芽处理，并在田间种植，以评价砧木的矮化效果。种植5年后，对树干横截面积和冠层体积的频率分布进行了RANKIT分析，发现34株矮化树和7株非矮化树存在两种重叠分布。这一比例与砧木矮化为单一显性基因的遗传是一致的，飞龙是‘杂合子’。‘飞龙’的两个形态特征，即弯曲的刺和扭曲的树干生长，与矮秆基因密切相关，或具有多效性。采用混合分离分析方法鉴定了3个与矮秆基因连锁的随机扩增多态性DNA标记。在40个纯合子和杂合子的同工酶和RFLP标记上，‘飞龙’与非矮秆橙品种完全相同，因此，‘飞龙’可能起源于一个非矮秆基因的突变体，自这次事件以来没有进行过有性重组。
'Flying Dragon' Poncirus trifoliata L. Raf. is a dwarfing rootstock for citrus. Inheritance of dwarfing ability was studied in a population of open-pollinated seedlings of 'Flying Dragon'. Molecular marker genotypes suggest that all seedlings originated from selfing. Progeny seedlings were budded with 'Cutter Valencia' orange and planted in the field to evaluate the dwarfing effect of the seedling rootstock. At 5 years after planting, rankit analysis of the frequency distributions of trunk cross-sectional area and canopy volume suggested the presence of two overlapping distributions of 34 dwarf trees and 7 nondwarf. This ratio is consistent with inheritance of rootstock dwarfing as a single dominant gene for which 'Flying Dragon' is' heterozygous. Two morphological characteristics of 'Flying Dragon', curved thorns and twisted trunk growth, were closely linked to, or pleiotropic effects of, the dwarfing gene. Bulked segregant analysis was used to identify three RAPD markers linked to the dwarfing gene. 'Flying Dragon' was identical to nondwarfing cultivars of trifoliate orange at 40 homozygous and heterozygous isozyme and RFLP markers; therefore, it is likely that 'Flying Dragon' originated as a mutant of a nondwarfing genotype and has not undergone sexual recombination since this event.