Imaging of Myocardial Ischemia-Reperfusion Injury Using Sodium [(18)F]Fluoride Positron Emission Tomography/Computed Tomography in Rats and Humans.
Imaging of Myocardial Ischemia-Reperfusion Injury Using Sodium [(18)F]Fluoride Positron Emission Tomography/Computed Tomography in Rats and Humans.
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DOI：
10.1177/1536012117704767
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发表时间：
2017-01-01
影响因子：
2.8
通讯作者：
Lee WW
中科院分区：
文献类型：
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作者：
Choi H;Han JH;Lim SY;Lee I;Cho YS;Chun EJ;Lee WW
使用氟化钠（Na[18F]F）的正电子发射断层扫描(PET)/计算机断层扫描（CT）已被证明是一种有前途的心肌梗死（MI）热点成像方式。我们研究了Na[18F]F在大鼠和人缺血再灌注损伤（IRI）中的摄取情况。对接受IRI手术的Sprague-Dawley大鼠进行氟化钠[18F] PET/CT检查，很容易发现IRI后梗死区域有明显的钠[18F]F摄取。钠[18F]氟摄取与2,3,5-三苯基- 2h -四氮氯化铵阴性染色结果相匹配，伴有心肌凋亡，并与钙阳性染色结果相关。此外，危险区域Na[18F]F摄取呈阴性。环孢素A （CysA）治疗降低了梗死区域18F的标准摄取值，并且CysA治疗也观察到梗死面积显着减小。在人类中，2例心肌梗死患者经皮冠状动脉介入治疗后，Na[18F]F PET/CT迅速显示Na[18F]F摄取增加。总之，本研究揭示了Na[18F]F PET/CT作为心肌IRI热点成像方式的潜在用途。
Positron emission tomography (PET)/computed tomography (CT) using sodium [18F]fluoride (Na[18F]F) has been proven to be a promising hot-spot imaging modality for myocardial infarction (MI). We investigated Na[18F]F uptake in ischemia–reperfusion injury (IRI) of rats and humans. Sodium [18F]fluoride PET/CT was performed in Sprague-Dawley rats that had IRI surgery, and it readily demonstrated prominent Na[18F]F uptake in the infarct area post-IRI. Sodium [18F]fluoride uptake was matched with negative 2,3,5-triphenyl-2H-tetrazolium chloride staining results, accompanied by myocardial apoptosis and associated with positive calcium staining results. Furthermore, area at risk was negative for Na[18F]F uptake. Cyclosporine A (CysA) treatment reduced standardized uptake value of 18F over the infarct area, and a significant decrease in infarct size was also observed by the CysA treatment. In humans, Na[18F]F PET/CT readily demonstrated increased Na[18F]F uptake in the 2 patients with MI post-percutaneous coronary intervention. In conclusion, this study sheds light on the potential utility of Na[18F]F PET/CT as a hot-spot imaging modality for myocardial IRI.