Changes in amino acid composition and nitrogen metabolizing enzymes in ripening fruits of Lycopersicon esculentum Mill
Changes in amino acid composition and nitrogen metabolizing enzymes in ripening fruits of Lycopersicon esculentum Mill
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DOI：
10.1016/s0168-9452(00)00342-3
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发表时间：
2000-10-08
期刊：
影响因子：
5.2
通讯作者：
Valle, EM
中科院分区：
文献类型：
--
作者：
Boggio, SB;Palatnik, JF;Valle, EM
番茄（Lycopersicon esculentum Mill.）在成熟过程中测定来自栽培品种Platense、Vollendung和Cherry的果实。结果表明，3个品种红果中谷氨酸含量显著增加。在这个阶段，樱桃品种具有最高的相对谷氨酸摩尔含量（52%）的所有分析的番茄果实品种。这些水果的氮同化酶活性的测量表明，谷氨酰胺合成酶（GS，EC 6.3.1.2）在果实成熟过程中的减少和伴随的增加，在NADH-谷氨酸脱氢酶（GDH，EC 1.4.1.3）和天冬氨酸氨基转移酶（EC 2.6.1.1）的活动。蛋白质提取物的Western印迹分析显示，虽然GS主要存在于绿色水果提取物中，但GDH几乎仅在红色水果提取物中观察到。这些结果表明，在番茄果实成熟过程中，GS和GDH之间的相互诱导模式。(C)2000爱思唯尔科学爱尔兰有限公司保留所有权利。
The free amino acid content of tomato (Lycopersicon esculentum Mill.) fruits from cultivars Platense, Vollendung and Cherry were determined during ripening. It was found that glutamate markedly increased in red fruits of the three cultivars under study. At this stage, the cv Cherry had the highest relative glutamate molar content (52%) of all the analyzed tomato fruit cultivars. Measurements of nitrogen-assimilating enzyme activities of these fruits showed a decrease in glutamine synthetase (GS, EC 6.3.1.2) during fruit ripening and a concomitant increase in NADH-glutamate dehydrogenase (GDH, EC 1.4.1.3) and aspartate aminotransferase (EC 2.6.1.1) activities. Western blot analysis of protein extracts revealed that while GS was principally present in green fruit extracts, GDH was almost exclusively observed in the extracts of red fruits. These results suggest a reciprocal pattern of induction between GS and GDH during tomato fruit ripening. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.