Bursting neurons command consummatory feeding behavior and coordinated visceral receptivity in the predatory mollusk Pleurobranchaea
Bursting neurons command consummatory feeding behavior and coordinated visceral receptivity in the predatory mollusk Pleurobranchaea
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爆发性神经元指挥捕食性软体动物侧鳃纲的完美摄食行为和协调内脏接受性
DOI：
10.1523/jneurosci.03-09-01791.1983
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发表时间：
1983
期刊：
影响因子：
64.8
通讯作者：
R. Gillette
中科院分区：
文献类型：
--
作者：
M. Gillette;R. Gillette
识别指挥复杂和协调的行为表达的中枢神经元位点提供了研究基本神经机制的机会，这些机制决定了特定行为是否表达以及在什么条件下表达。先前在侧鳃亚纲的分离的神经系统中显示，颊神经节的双侧配对的腹侧白色细胞（VWC）在长时间的内源性爆发发作期间驱动神经网络中的循环运动输出，该神经网络记录在到颊块的切断神经根中（Gillette，R.，M.联合Gillette和W. 03 The Dog（1978）Neurosci. 4：1210，（1980）J. Neurophysiol. 43：669 - 685）。这种爆发发作也可由CAMP及其激动剂诱导（Gillette，M. U.、R. Gillette和W. 03 The Dog（1978）Neurosci. 4：1209; Gillette，R.，M.联合Gillette和W. J.Davis（1982）J.Comp.Physiol.146：461 - 470）。由VWC驱动的活动的行为意义和它们的正常外在激活剂的性质一直是推测的主题（Gillette，R.，M.联合Gillette和W. 03 The Dog（1978）Neurosci. 4：1210，（1980）J. Neurophysiol. 43：669 - 685; McClellan，A. D. 1980年博士学位论文，凯斯西储大学; Croll，R. P.，和w. J.Davis（1982）J.Comp.Physiol.147：143 - 153），但仍有待通过行为期间细胞活性的精确规格来建立。在前半部分动物制备中，其以适当的行为对食欲和呕吐刺激作出反应，食欲（食物）刺激，而不是呕吐物质，激活先前静止的VWC。这种激活的特征是膜电位缓慢去极化，伴有短节奏爆发（5至8个尖峰）;它在长时间爆发事件中达到高潮（持续去极化和3至5 Hz的尖峰持续0.7至5.0 min）。鱿鱼匀浆对VWC的激活与CAMP及其激动剂对细胞的激活在细节上类似（M. Gillette，提交出版）。食欲物质可以通过刺激口腔粘膜激活VWC，但它们在口腔中最有效。在鱿鱼匀浆激活的长时间爆发发作期间，在尖峰明显扩大的时候，VWC开始驱动齿舌的极端缩回/伸长周期，这是摄食行为的特征。这与早期的工作一致，早期的工作证明了爆发期间的尖峰加宽对于在孤立的神经系统中驱动运动输出是必须的（Gillette，R.，M.联合Gillette和W. J. Davis（1980）J. Neurophysiol. 43：669 - 685）。同时，VWC通过其外周轴突的协同作用，刺激括约肌样颊食管收缩的打开，以及食道（食物储存器官）的明显纵向收缩和环形扩张。在长时间的VWC爆发发作期间，固体食物材料通过齿舌的极端缩回/前伸运动逐渐从颊腔移动到该缩短的食管中，证实了该运动模式的摄食性质。从未观察到应用于口腔面纱或口腔的催吐物质产生广泛或延长的尖峰活性，也未观察到它们导致VWC破裂。催吐刺激产生的周期性口腔质量运动明显不同于喂养，我们解释为1这项工作得到了华盛顿美国大学的研究资助，用于一些研究中使用的设施;和哲学学会M。联合G.和国家科学基金会，特别是维拉·弗雷特博士，因为她在软体动物生物学方面的早期指导。授予R BNS-79 - 18329。G.感谢C博士。L. Prosser，J.伦敦，* 通信应向谁，在D部门。绿色，M医生。P.莫尔斯和F. Delcomyn为有益的讨论生理学和生物物理学，524伯里尔大厅，伊利诺伊大学，和关键的阅读的手稿;星期五港实验室，厄巴纳，IL 61801。
The identification of central neuronal loci that command complex and coordinated behavioral expression offers the opportunity to study basic neural mechanisms that determine whether and under what conditions a specific behavior is expressed. It was previously shown in the isolated nervous system of Pleurobranchaea that the bilaterally paired ventral white cells (VWCs) of the buccal ganglion drive cyclic motor output in the neural network recorded in cut nerve roots to the buccal mass during prolonged endogenous burst episodes (Gillette, R., M. U. Gillette, and W. J. Davis (1978) Sot. Neurosci. Abstr. 4: 1210, (1980) J. Neurophysiol. 43: 669-685). Such burst episodes are also inducible by CAMP and its agonists (Gillette, M. U., R. Gillette, and W. J. Davis (1978) Sot. Neurosci. Abstr. 4: 1209; Gillette, R., M. U. Gillette, and W. J. Davis (1982) J. Comp. Physiol. 146: 461-470). The behavioral significance of the activity driven by the VWCs and the nature of their normal extrinsic activators has been a subject for speculation (Gillette, R., M. U. Gillette, and W. J. Davis (1978) Sot. Neurosci. Abstr. 4: 1210, (1980) J. Neurophysiol. 43: 669-685; McClellan, A. D. (1980) Ph.D. thesis, Case Western Reserve University; Croll, R. P., and W. J. Davis (1982) J. Comp. Physiol. 147: 143-153) but has remained to be established by exact specification of cell activity during behavior. In an anterior hemi-animal preparation, which responds to appetitive and emetic stimuli with appropriate behavior, appetitive (food) stimuli, but not emetic substances, activate the previously quiescent VWCs. This activation is characterized by slow depolarization of the membrane potential accompanied by short rhythmic bursts (5 to 8 spikes); it culminates in a prolonged burst episode (sustained depolarization and spiking at 3 to 5 Hz for 0.7 to 5.0 min). Activation of a VWC by squid homogenate resembles in detail the activation of the cell by CAMP and its agonists (M. Gillette, submitted for publication). Appetitive substances can activate the VWCs via stimulation of the oral veil, but they are most effective in the buccal cavity. During the prolonged burst episode activated by squid homogenate, at the time the spike has broadened appreciably, the VWCs begin to drive the extreme retraction/protraction cycles of the radula that are characteristic of feeding behavior. This is consistent with earlier work which demonstrated that spike broadening during the burst is obligatory for driving motor output in the isolated nervous system (Gillette, R., M. U. Gillette, and W. J. Davis (1980) J. Neurophysiol. 43: 669-685). Simultaneously, in concerted action via their peripheral axons, the VWCs stimulate opening of a sphincter-like buccal-esophageal constriction, as well as marked longitudinal contraction and circular dilation of the esophagus, which is a food storage organ. During prolonged VWC burst episodes, solid food material is progressively moved from the buccal cavity into this shortened esophagus by the extreme retraction/protraction movements of the radula, confirming the ingestive nature of this motor pattern. Emetic substances applied to the oral veil or buccal cavity were never observed to produce extensive or prolonged spike activity, nor did they result in bursting of the VWCs. Emetic stimuli produce cyclic buccal mass movements distinctly different from feeding which we interpret as 1 This work was supported by a research grant from the American University of Washington for facilities used in some studies; and Philosophical Society to M. U. G. and National Science Foundation especially Dr. Vera Fretter for her early guidance in molluscan biology. Grant BNS-79-18329 to R. G. We thank Dr. C. L. Prosser, J. London, * To whom correspondence should be addressed, at Department of D. Green, Dr. M. P. Morse, and Dr. F. Delcomyn for helpful discussion Physiology and Biophysics, 524 Burrill Hall, University of Illinois, and critical reading of the manscript; Friday Harbor Laboratories, Urbana, IL 61801.