MicroRNA expression profiling of the developing murine molar tooth germ and the developing murine submandibular salivary gland
MicroRNA expression profiling of the developing murine molar tooth germ and the developing murine submandibular salivary gland
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DOI：
10.1016/j.archoralbio.2008.01.014
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发表时间：
2008-07-01
影响因子：
3
通讯作者：
Osmundsen, Harald
中科院分区：
文献类型：
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作者：
Jevnaker, Anne-Marthe;Osmundsen, Harald
利用微阵列技术，在小鼠下颌第一磨牙牙胚和右下颌下唾液腺发育过程中的选定时间（E15.5，P0和P5）建立了miRNA表达谱。使用实时PCR验证微阵列数据，也有助于9个选定的miRNA的RT-PCR谱。总体而言，微阵列数据和实时PCR数据之间有很好的一致性。此外，我们还研究了胎儿和成人肝脏的miRNA表达谱，发现与已发表的数据一致。在牙胚和唾液腺中检测到多达88种不同的miRNAs。在所有检测的组织中，miRNA表达是高度动态的; miRNA谱随发育时间而广泛变化。此外，一些miRNA的表达是组织特异性的。尝试使用miRGate软件对miRNAs簇进行生物信息学分析，结果表明miRNAs参与调控基本发育过程，例如，上皮细胞增殖、中胚层细胞命运决定和唾液腺形态发生。(C)2008爱思唯尔有限公司保留所有权利。
Using microarrays, miRNA expression profiles have been established at selected times during development (E15.5, P0 and P5) of the murine first molar mandibular tooth germ and the right submandibular salivary gland (E15.5, P0, P5 and P25). Microarray data was validated using real-time PCR, also facilitating RT-PCR profiling of nine selected miRNAs. In general, good agreement between microarray data and real-time PCR data was found. Further, miRNA expression profiles of foetal and adult liver were also investigated, and found to agree with published data. In tooth germ and salivary gland up to 88 different miRNAs were detected. In all tissues examined miRNA expression was highly dynamic; miRNA profiles changing extensively with time of development. Additionally, the expression of some miRNAs was tissue-specific. Bioinformatic analysis of clusters of miRNAs was attempted using the miRGate software, the results suggesting miRNAs to be involved in the regulation of essential developmental processes, e.g., epithelical cell proliferation, mesodermal cell fate determination and salivary gland morphogenesis. (C) 2008 Elsevier Ltd. All rights reserved.