The uptake of PLGA micro or nanoparticles by macrophages provokes distinct in vitro inflammatory response
The uptake of PLGA micro or nanoparticles by macrophages provokes distinct in vitro inflammatory response
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DOI：
10.1016/j.intimp.2011.05.014
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发表时间：
2011-10-01
影响因子：
5.6
通讯作者：
Faccioli, Lucia H.
中科院分区：
文献类型：
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作者：
Nicolete, Roberto;dos Santos, Daiane F.;Faccioli, Lucia H.
由PLGA产生的可生物降解的微米/纳米颗粒最近由于其临床证明的生物相容性而引起关注，特别是用于免疫目的。这些聚合物微粒递送系统能够呈递抗原并激活体液和细胞应答。许多研究已经讨论了这些颗粒的理想尺寸有助于产生不同类型的免疫反应。然而，这些研究没有证明没有任何包封的生物活性物质的微米或纳米颗粒在摄取过程后对吞噬细胞的影响。在这种情况下，本研究的目的是分析J774小鼠巨噬细胞在颗粒摄取后的体外炎症行为，因为纳米/微粒本身可以不同地激活吞噬细胞，使用或不使用适当的受体，诱导不同的炎症反应。采用O/W型乳化溶剂萃取-蒸发法制备纳米粒子。我们测定了它们的直径，zeta电位和形态。还分析了J774鼠“巨噬细胞样”细胞对荧光颗粒的摄取。为了评估这些细胞在微米或纳米颗粒摄取后的体外炎症特征，我们通过共聚焦显微镜进行了NF-κ B易位测定，并且还确定了由颗粒引起的促炎细胞因子产生。(C)2011爱思唯尔有限公司版权所有。
Biodegradable micro/nanoparticles generated from PLGA have recently attracted attention due to their clinically proven biocompatibility, especially for immunization purposes. These polymeric particulate delivery systems are able to present antigens and activate both humoral and cellular responses. Many studies have discussed the ideal size of these particles in contributing to the generation of the different types of immune response. However, these studies do not demonstrate the effect of micro or nanoparticles, without any encapsulated bioactive, on phagocytic cells after the uptake process. In this context, the aim of this study was to analyze the in vitro inflammatory behavior of J774 murine macrophages after particles' uptake, since nano/microparticles per se can differently activate phagocytic cells, using or not appropriate receptors, inducing distinct inflammatory responses. An o/w emulsion solvent extraction-evaporation method was chosen to prepare the particles. We determined their diameters, zeta potential and morphology. Fluorescent particles' uptake by J774 murine "macrophage-like" cells was also analyzed. To evaluate the in vitro inflammatory profile of these cells after micro or nanoparticles' uptake, we conducted NF-kappa B translocation assay by confocal microscopy and also determined the pro-inflammatory cytokines production provoked by the particles. (C) 2011 Elsevier B.V. All rights reserved.