Amplification of human immuno-deficiency virus production from a virus-producing cell line in culture at high cell density.
Amplification of human immuno-deficiency virus production from a virus-producing cell line in culture at high cell density.
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从高细胞密度培养的病毒产生细胞系中扩增人类免疫缺陷病毒的产生。
DOI：
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
S. Kato
中科院分区：
文献类型：
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作者：
K. Ikuta;H. Imai;S. Ueda;S. Suehiro;N. Yamamoto;S. Kato
测定从产生人类免疫缺陷病毒（HIV）的MOLT-4细胞（MOLT-4/HIV HTLV-III）释放到培养液中的感染性病毒的量，作为培养条件和细胞密度的函数。3天后，在高细胞密度培养的非生长细胞液中，每个细胞的日传染性病毒产量比在低细胞密度培养的生长细胞的日累积产量高约15倍。SDS-PAGE分析也显示了主要gag蛋白p24数量的类似差异。
The amount of infectious virus released into the culture fluid from human immunodeficiency virus (HIV)-producing MOLT-4 cells (MOLT-4/HIV HTLV-III) was measured as a function of the culture conditions and cell density. After 3 days, the daily yield of infectious virus per cell in the fluid of non-growing cells cultured at high cell density was about 15 times higher than the accumulated yield from growing cells cultured at low cell density. SDS-PAGE analysis also showed a similar difference in the amounts of major gag protein p24.
浅蓝菌素抑制莫洛尼鼠白血病病毒 gag 和 env 编码的前体多蛋白的裂解。
影响因子：
3.7
作者：
Ikuta,K;Luftig,RB
通讯作者：
Luftig,RB