Expression and immunohistochemical localization of aquaporin-1 in male reproductive organs of the mouse
Expression and immunohistochemical localization of aquaporin-1 in male reproductive organs of the mouse
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DOI：
10.1111/j.1439-0264.2007.00827.x
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发表时间：
2008-02-01
影响因子：
0.9
通讯作者：
Li, X. J.
中科院分区：
文献类型：
--
作者：
Lu, D. Y.;Li, Y.;Li, X. J.
水通道蛋白-1（AQP-1）是一种具有六个跨膜结构域的蛋白质，主要负责水的转运。本研究采用逆转录聚合酶链反应（RT-PCR）和蛋白质印迹法（Western blotting）检测AQP-1 mRNA和蛋白在成年小鼠睾丸、附睾、输精管、前列腺腹侧部和精囊中的表达，并采用免疫组织化学方法检测AQP-1的细胞定位。RT-PCR结果显示，AQP-1 mRNA在所有器官中均有表达。Western印迹显示29-kDa条带和35- 45-kDa条带，对应于这些器官中的非糖基化和/或糖基化AQP-1。免疫组化结果显示，AQP-1主要定位于睾丸网、输精管、前列腺腹侧精囊上皮细胞及近端和远端输出管无纤毛细胞的质膜上。然而，AQP-1是不存在的生精细胞内衬生精上皮和精子在管腔的远端输出管。这些发现为AQP-1在男性生殖器官中的表达提供了有价值的信息，并表明AQP-1参与水运输以调节男性生殖生理中的水稳态。
Aquaporin-1 (AQP-1), a six-transmembrane domain protein, is found to be responsible for water transport. The purpose of this study was to investigate the expression of AQP-1 mRNA and protein in the testis, epididymis, vas deferens, ventral prostate, and seminal vesicle from mature mice by using reverse transcription polymerase chain reaction (RT-PCR) and Western blotting, and the cellular localization of AQP-1 by immunohistochemistry. RT-PCR revealed that AQP-1 mRNA was expressed in all organs we examined. Western blotting displayed a 29-kDa band and a 35- to 45-kDa band corresponding to non-glycosylated and/or glycosylated AQP-1 in those organs. The immumohistochemical evidence showed that AQP-1 was mainly located on the plasma membrane of epithelial cells of the rete testis, vas deferens, ventral prostate seminal vesicle and the non-ciliated cells of the proximal an distal efferent ducts. However, AQP-1 was absent from spermatogenic cells lining the seminiferous epithelium and from the spermatozoa in the lumen of the distal efferent duct. These findings provide valuable information on the expression of AQP-1 in male reproductive organs and suggest that AQP-1 is involved in water transport to regulate water homeostasis in male reproductive physiology.