TECHNIQUES FOR THE OBSERVATION AND MEASUREMENT OF RED-BLOOD-CELL VELOCITY IN VESSELS OF THE GUINEA-PIG COCHLEA
TECHNIQUES FOR THE OBSERVATION AND MEASUREMENT OF RED-BLOOD-CELL VELOCITY IN VESSELS OF THE GUINEA-PIG COCHLEA
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DOI：
10.1016/0378-5955(87)90012-8
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发表时间：
1987-01-01
期刊：
影响因子：
2.8
通讯作者：
NUTTALL, AL
中科院分区：
文献类型：
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作者：
NUTTALL, AL
荧光技术与活体显微镜的结合为研究耳蜗血微循环提供了一种强有力的方法。在本研究中，将高分子右旋糖苷偶联的异硫氰酸荧光素添加到血浆中，以增强豚鼠耳蜗镜下图像中流动的血液的视觉对比度。从血管的视频图像中获得的光度学信号，通过使用互相关算法来提取图像运动特征的时延，提供了一种连续测量红细胞速度的方法。或者，将少量荧光标记的红细胞(RBC)添加到血管体积中，作为全血流量的自然指示器。通过视频光度检测细胞在电视图像中的两个预定位置之间通过所需的时间来测量这些细胞的速度。红细胞可以通过将荧光色素化学结合到细胞膜上或通过在细胞内负载惰性荧光色素来产生荧光。标记的红细胞提供了一种在光学对比度极差的毛细血管中测定血流速度的方法，这种情况通常适用于相对较厚的组织，如膜迷路的侧壁。
Fluorescence techniques combined with intravital microscopy provide a powerful approach to the study of cochlear blood microcirculation. In the current study, fluorescein isothiocyanate conjugated to high molecular weight dextrans was added to plasma to enhance the visual contrast of flowing blood in microscopic images from the guinea pig cochlea. Photometric signals, obtained from video pictures of the blood vessels, provided a means to continuously measure red cell velocity by using crosscorrelation algorithms to extract the time delay for moving features of the image. Alternatively, a small amount of fluorescently-labeled red blood cells (RBCs) were added to the vascular volume to serve as natural indicators of whole blood flow. The speed of these cells was measured by video photometric detection of the time required for the cells to pass between two predetermined positions in the television image. RBCs can be made fluorescent by chemical bonding of a fluorochrome to the cell membrane or by internal loading of the cell with an inert fluorochrome. Labeled RBCs provide a means to determine blood velocity in capillaries having extremely poor optical contrast, a situation which is generally the case for relatively thick tissues such as the lateral wall of the membranous labyrinth.