Single particle tracking reveals biphasic transport during nanorod magnetophoresis through extracellular matrix
Single particle tracking reveals biphasic transport during nanorod magnetophoresis through extracellular matrix
复制标题
DOI：
10.1039/c4sm00611a
复制
发表时间：
2014-01-01
期刊：
影响因子：
3.4
通讯作者：
Superfine, R.
中科院分区：
文献类型：
--
作者：
Mair, L. O.;Superfine, R.
磁性药物靶向已被提议作为有效地将药物靶向肿瘤的手段。然而，细胞外基质（ECM）仍然是通过肿瘤体积进行长程磁泳运输的重要障碍。虽然纳米粒子磁泳的整体测量已有报道，但对磁泳输运的单粒子水平理解仍然很广泛。我们基于单粒子观察通过 ECM 量化纳米棒磁泳。我们发现，尽管磁力较小，但直径较小的颗粒通过 ECM 可以获得较大的速度。此外，还阐明了两个有趣的动态。首先，18 nm 直径的纳米棒在静态场磁泳过程中通过 ECM 经历双峰粘滑运动，而 55 nm 或 200 nm 直径的纳米棒则没有观察到类似的双峰传输。其次，较小的颗粒相对于磁场的方向角存在较大偏差。这项工作阐明了纳米颗粒通过复杂的多孔生物材料传输的重要动力学，这些动力学在整体测量过程中可能被忽视。
Magnetic drug targeting has been proposed as a means of efficiently targeting drugs to tumors. However, the extracellular matrix (ECM) remains a significant barrier to long-range magnetophoretic transport through the tumor volume. While ensemble measurements of nanoparticle magnetophoresis have been reported, a single particle level understanding of magnetophoretic transport remains at large. We quantify nanorod magnetophoresis through ECM based on single particle observations. We find that smaller diameter particles achieve larger velocities through ECM despite experiencing smaller magnetic forces. Additionally, two interesting dynamics are elucidated. First, 18 nm diameter nanorods experience bimodal stick-slip motion through ECM during static field magnetophoresis, while similar bimodal transport is not observed for 55 nm nor 200 nm diameter nanorods. Second, smaller particles experience larger deviations in their orientation angle with respect to the magnetic field. This work elucidates important dynamics of nanoparticle transport through complex, porous biomaterials that may go unnoticed during ensemble measurements.