Identification and expression of an autosomal paralogue of ribosomal protein S4, X-linked, in mice: potential involvement of testis-specific ribosomal proteins in translation and spermatogenesis.
Identification and expression of an autosomal paralogue of ribosomal protein S4, X-linked, in mice: potential involvement of testis-specific ribosomal proteins in translation and spermatogenesis.
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DOI：
10.1016/j.gene.2013.02.040
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发表时间：
2013-05
期刊：
影响因子：
3.5
通讯作者：
Yoshihiko Sugihara;Eiji Sadohara;Kahori Yonezawa;Mariko Kugo;Kenzi Oshima;T. Matsuda;D. Nadano
中科院分区：
文献类型：
--
作者：
Yoshihiko Sugihara;Eiji Sadohara;Kahori Yonezawa;Mariko Kugo;Kenzi Oshima;T. Matsuda;D. Nadano
最近的研究揭示了真核细胞质核糖体结构的异质性，包括蛋白质组成的差异。有人提出，这种异质性或特殊的核糖体通过选择性 mRNA 翻译有助于组织发育和体内平衡，尽管这在很大程度上仍不清楚。我们之前对啮齿动物核糖体的蛋白质组学调查发现了睾丸特异性核糖体蛋白 L10 样和 L39 样，它们分别是 X 连锁核糖体蛋白 L10 和 L39 的旁系同源物。我们假设啮齿动物睾丸为检查核糖体异质性可能的功能重要性提供了一个很好的模型。在本研究中，在小鼠睾丸中鉴定出了 X 连锁核糖体蛋白 S4 的新旁系同源物。编码该旁系同源物的基因是常染色体、无内含子并且主要在睾丸中表达。看来该旁系同源物作为核糖体的组成部分包含在多核糖体中。尽管这些特性与核糖体蛋白 L10 样和 L39 样相似，但这种 S4 旁系同源物和 L10 样在生精细胞中表现出部分不同的表达模式。这些发现的讨论涉及哺乳动物中编码核糖体蛋白 S4 旁系同源物的基因的独特进化，以及精子发生过程中活性核糖体中睾丸特异性核糖体蛋白旁系同源物的重要性。
Recent studies have revealed heterogeneity in the structure of eukaryotic cytoplasmic ribosomes, including a difference in protein composition. It has been proposed that this heterogeneity, or the specialized ribosome, contributes to tissue development and homeostasis through selective mRNA translation, although this remains largely unclear. Our previous proteomic survey of rodent ribosomes found the testis-specific ribosomal proteins L10-like and L39-like, which are paralogues of the X-linked ribosomal proteins L10 and L39, respectively. We have hypothesized that the rodent testis provides a good model for examining the possible functional importance of ribosome heterogeneity. In the present study, a new paralogue of X-linked ribosomal protein S4 has been identified in the mouse testis. The gene encoding this paralogue was autosomal, intronless and expressed predominantly in the testis. It appeared that this paralogue was included in polysomes as a component of the ribosome. Although these properties were similar to those of the ribosomal proteins L10-like and L39-like, this S4 paralogue and L10-like showed partially different expression patterns in spermatogenic cells. These findings are discussed in relation to the unique evolution of genes encoding a paralogue of ribosomal protein S4 in mammals and to the significance of testis-specific paralogues of ribosomal proteins in active ribosomes during spermatogenesis.