Transgenic Hydra allow in vivo tracking of individual stem cells during morphogenesis
Transgenic Hydra allow in vivo tracking of individual stem cells during morphogenesis
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DOI：
10.1073/pnas.0510163103
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发表时间：
2006-04-18
影响因子：
11.1
通讯作者：
Bosch, TCG
中科院分区：
文献类型：
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作者：
Wittlieb, J;Khalturin, K;Bosch, TCG
了解发育的进化在很大程度上依赖于对系统发育古老生物的研究。蛇类动物，如九头蛇，已经成为这些研究的有吸引力的模式生物。然而，尽管进行了长期的努力，仍无法产生稳定的转基因动物，这严重限制了基因的功能分析。在这里，我们报道了用胚胎显微注射的方法高效地产生转基因水母细胞系。其中一个表达绿色荧光蛋白的转基因系显示了单个内胚层上皮细胞在形态发生过程中的高运动性。我们期望转基因的九头蛇将成为在后生动物树的基础上剖析发育的分子机制的重要工具。
Understanding the evolution of development in large part relies on the study of phylogenetically old organisms. Cnidarians, such as Hydra, have become attractive model organisms for these studies. However, despite long-term efforts, stably transgenic animals could not be generated, severely limiting the functional analysis of genes. Here we report the efficient generation of transgenic Hydra lines by embryo microinjection. One of these transgenic lines expressing EGFP revealed remarkably high motility of individual endodermal epithelial cells during morphogenesis. We expect that transgenic Hydra will become important tools to dissect the molecular mechanisms of development at the base of the Metazoan tree.