Tumor core biopsies adequately represent immune microenvironment of high-grade serous carcinoma
Tumor core biopsies adequately represent immune microenvironment of high-grade serous carcinoma
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DOI：
10.1038/s41598-019-53872-1
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发表时间：
2019-11-26
影响因子：
4.6
通讯作者：
Sood, Anil K.
中科院分区：
文献类型：
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作者：
Lara, Olivia D.;Krishnan, Santhoshi;Sood, Anil K.
肿瘤微环境(TME)在不同癌症类型中的预后和治疗价值是人们主要关注的问题。TME的特征通常依赖于一个具有代表性的小组织样本。然而，目前尚不清楚这种样本是否足以评估TME的组成部分。在这里，我们用免疫组织化学(IHC)染色和7色多重染色检测了26例高级别浆液性卵巢癌220个组织核心中的CD8(分化簇8)、CD68、PD-L1(程序性死亡配体1)、CD34、FAP(成纤维细胞激活蛋白)和细胞角蛋白。根据活检的数量和位置(中心肿瘤与周围肿瘤)，比较较大的肿瘤标本和较小的核心活检组织。我们的分析发现，在较大的肿瘤和较小的核心中，标记特异性细胞亚群之间的相关性在两个核心活组织检查中更强，并且不会随着额外的活组织检查而进一步加强。此外，无论活检是在原始肿瘤样本的中心还是边缘进行，这种相关性都是一致的。这些发现可能会对临床试验中生物标志物检测的纵向评估产生重大影响。
The prognostic and therapeutic value of the tumor microenvironment (TME) in various cancer types is of major interest. Characterization of the TME often relies on a small representative tissue sample. However, the adequacy of such a sample for assessing components of the TME is not yet known. Here, we used immunohistochemical (IHC) staining and 7-color multiplex staining to evaluate CD8 (cluster of differentiation 8), CD68, PD-L1 (programmed death-ligand 1), CD34, FAP (fibroblast activation protein), and cytokeratin in 220 tissue cores from 26 high-grade serous ovarian cancer samples. Comparisons were drawn between a larger tumor specimen and smaller core biopsies based on number and location (central tumor vs. peripheral tumor) of biopsies. Our analysis found that the correlation between marker-specific cell subsets in larger tumor versus smaller core was stronger with two core biopsies and was not further strengthened with additional biopsies. Moreover, this correlation was consistently strong regardless of whether the biopsy was taken at the center or at the periphery of the original tumor sample. These findings could have a substantial impact on longitudinal assessment for detection of biomarkers in clinical trials.