Does Pregnancy Have an Impact on the Subgingival Microbiota?
Does Pregnancy Have an Impact on the Subgingival Microbiota?
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DOI：
10.1902/jop.2009.080012
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发表时间：
2009-01-01
影响因子：
4.3
通讯作者：
Persson, G. Rutger
中科院分区：
文献类型：
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作者：
Adriaens, Laurence A.;Alessandri, Regina;Persson, G. Rutger
背景：我们调查了20名连续18年未接受牙科护理的孕妇在妊娠期间的临床和龈下微生物变化。方法：采用棋盘DNA-DNA杂交法对妊娠12周、28周、36周和产后4~6周的37种细菌样本进行处理。结果：产后12周和产后4~6周的平均探诊出血率分别为40.1%±18.2%和27.4%±12.5%。相应的微生物检测部位的平均BOP为15%(12周)和21%(产后；无统计学意义)。从第12周到产后，细菌总数减少(P<；0.01)。随着时间的推移，发现奈瑟氏菌粘膜的细菌计数增加(P<；0.001)。较低的菌落数(P<；0.001)有：微囊藻、微囊藻、真核真细菌、核梭杆菌、多形梭杆菌、桶细毛藻、微小拟单胞菌(原微型消化链球菌或微型微单胞菌)、中间普罗特氏菌、产黑前杆菌、金黄色葡萄球菌、链球菌、中间链球菌、变形链球菌、口腔细毛杆菌、血链球菌、新生葡萄球菌和细小葡萄球菌。在怀孕12周到28周之间没有发生变化。伴生放线杆菌(前身为放线菌伴生放线杆菌)、牙龈卟啉单胞菌、连翘坦纳氏菌(前身为连翘叶蝉)和齿密螺旋体的数量没有变化。12周时牙龈假单胞菌和连翘的数量与牙周炎有关(P<；0.001)。结论：与牙周炎相关的细菌水平没有改变。在12周时，牙周炎和连翘数量与BOP相关。从12周到产后，37种细菌中有17种出现了下降。仅粘膜N计数增加。《月经周期》2009；80：72-81。
Background: We investigated clinical and subgingival microbiologic changes during pregnancy in 20 consecutive pregnant women >= 18 years not receiving dental care.Methods: Bacterial samples from weeks 12, 28, and 36 of pregnancy and at 4 to 6 weeks postpartum were processed for 37 species by checkerboard DNA-DNA hybridization. Clinical periodontal data were collected at week 12 and at 4 to 6 weeks postpartum, and bleeding on probing (BOP) was recorded at sites sampled at the four time points.Results: The mean BOP at week 12 and postpartum was 40.1% +/- 18.2% and 27.4% +/- 12.5%, respectively. The corresponding mean BOP at microbiologic test sites was 15% (week 12) and 21 % (postpartum; not statistically significant). Total bacterial counts decreased between week 12 and postpartum (P < 0.01). Increased bacterial counts over time were found for Neisseria mucosa (P < 0.001). Lower counts (P < 0.001) were found for Capnocytophaga ochracea, Capnocytophaga sputigena, Eubacterium saburreum, Fusobacterium nucleatum naviforme, Fusobacterium nucleatum polymorphum, Leptotrichia buccalls, Paruimonas micra (previously Peptostreptococcus micros or Micromonas micros), Preuotella intermedia, Preuotella melaninogenica, Staphylococcus aureus, Streptococcus anginosus, Streptococcus intermedius, Streptococcus mutans, Streptococcus oralis, Streptococcus sanguinis, Selenomonas noxia, and Veillonella paruula. No changes occurred between weeks 12 and 28 of pregnancy. Counts of Aggregatibacter actinomycetemcomitans (previously Actinobacillus actinomycetemcomitans), Porphyromonas gingivalis, Tannerella forsythia (previously T. forsythensis), and Treponema denticola did not change. Counts of P. gingiualis and T. forsythia at week 12 were associated with gingivitis (P < 0.001).Conclusions: Subgingival levels of bacteria associated with periodontitis did not change. P. gingiualis and T. forsythia counts were associated with BOP at week 12. A decrease was found in 17 of 37 species from week 12 to postpartum. Only counts of N. mucosa increased. J Periodontol 2009;80:72-81.