Comparison of site-specific injections into the basal forebrain on water maze and radial arm maze performance in the male rat after immunolesioning with 192 IgG saporin
Comparison of site-specific injections into the basal forebrain on water maze and radial arm maze performance in the male rat after immunolesioning with 192 IgG saporin
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DOI：
10.1016/s0166-4328(97)81112-6
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发表时间：
1996-12-01
影响因子：
2.7
通讯作者：
Gunther, KL
中科院分区：
文献类型：
--
作者：
Dornan, WA;McCampbell, AR;Gunther, KL
在这项研究中，我们研究了将 192 IgG 肥皂草素注射到内侧间隔区 (MSA) 或大细胞基底核 (NBM) 以及联合注射到 MSA 和 NBM 对雄性大鼠水迷宫和径向臂迷宫表现的影响。本研究的结果揭示了向基底前脑注射 192 Ige 皂草素对大鼠空间学习两项任务表现的影响之间的分离。与对照相比，将 192 IgG 肥皂草素双侧注射到 NBM、MSA 或组合的 MSA/NBM 中未能破坏水迷宫的性能。相比之下，将 192 IgG 肥皂草素注射到 MSA、NBM 或 MSA/NBM 中会导致径向臂迷宫任务出现轻微损伤。然而，总体而言，与使用胆碱能基底前脑选择性较低的损伤所报告的空间学习缺陷相比，本研究中观察到的空间学习中断相对轻微。因此，这项研究的结果表明，基底前脑胆碱能传递的选择性减少本身不足以解释大鼠空间学习中观察到的功能障碍。虽然我们的数据确实支持使用 192 IgG 皂草素作为基底前脑的选择性胆碱能毒素，但他们进一步表明，在基底前脑 192 IgG 皂草素损伤并结合其他神经递质系统损伤后对大鼠的空间学习进行评估，可能是阐明与阿尔茨海默氏病认知缺陷相关的神经病理学机制的更可行的方法。
In this study, we investigated the effects of 192 IgG saporin injections into the medial septal area (MSA), or nucleus basalis magnocellularis (NBM), and combined injections into the MSA and NBM, on water maze and radial arm maze performance in the male rat. The results of the present study reveal a dissociation between the effects of 192 Ige saporin injections into the basal forebrain on the performance of two tasks of spatial learning in the rat. Bilateral injections of 192 IgG saporin into the NBM, MSA or combined MSA/NBM failed to disrupt water maze performance when compared to controls. In contrast, injections of 192 IgG saporin into the MSA, NBM or MSA/NBM induced mild impairments on a radial arm maze task. Overall, the disruption of spatial learning observed in this study was, however, relatively mild compared to deficits in spatial learning reported using less selective lesions of the cholinergic basal forebrain. Consequently, the results of this study suggest that a selective reduction in cholinergic transmission in the basal forebrain is, by itself, insufficient to account for the functional impairments observed in spatial learning in the rat. Although our data do support the use of 192 IgG saporin as a selective cholinergic toxin in the basal forebrain, they further suggests that assessment of spatial learning in the rat following 192 IgG saporin lesions of the basal forebrain in combination with lesions to other neurotransmitter systems, may be a more viable approach to the elucidation of the neuropathological mechanisms that are associated with the cognitive deficits seen in Alzheimer's disease.