Arabidopsis EF-Tu receptor enhances bacterial disease resistance in transgenic wheat
Arabidopsis EF-Tu receptor enhances bacterial disease resistance in transgenic wheat
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DOI：
10.1111/nph.13356
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发表时间：
2015-04-01
期刊：
影响因子：
9.4
通讯作者：
Ridout, Christopher J.
中科院分区：
文献类型：
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作者：
Schoonbeek, Henk-jan;Wang, Hsi-Hua;Ridout, Christopher J.
模式识别受体(PRRs)对病原体(或微生物)相关分子模式(PAMPs/MAMPs)的感知是植物天然免疫的重要组成部分。拟南芥PRR EF-Tu受体(EFR)识别细菌PAMP延伸因子Tu(EF-Tu)及其衍生的多肽elf18。以前的工作表明，在茄科植物中转基因表达AtEFR具有Elf18反应性和广谱细菌抗病能力。在这项研究中，我们建立了一套生物检测方法来研究小麦中PAMP触发免疫(PTI)的激活。我们获得了由组成型水稻肌动蛋白启动子驱动的表达AtEFR的转基因小麦(Triticum Aestivum)植株，并测试了它们对elf18的响应。我们表明，在小麦中转基因表达AtEFR可以识别elf18，这是通过诱导免疫标记基因和胼胝质沉积来衡量的。转EFR基因小麦品系在受到谷类病原菌假单胞菌侵染后，病斑面积减小，细菌繁殖减少。这些结果表明AtEFR可以成功地从双子叶植物转移到单子叶植物，进一步揭示了免疫信号通路在这些遥远的门之间是保守的。随着新的PRR被鉴定出来，它们在植物家族之间的转移是提高作物对病原菌抗性的有用策略。
Perception of pathogen (or microbe)-associated molecular patterns (PAMPs/MAMPs) by pattern recognition receptors (PRRs) is a key component of plant innate immunity. The Arabidopsis PRR EF-Tu receptor (EFR) recognizes the bacterial PAMP elongation factor Tu (EF-Tu) and its derived peptide elf18. Previous work revealed that transgenic expression of AtEFR in Solanaceae confers elf18 responsiveness and broad-spectrum bacterial disease resistance. In this study, we developed a set of bioassays to study the activation of PAMP-triggered immunity (PTI) in wheat. We generated transgenic wheat (Triticum aestivum) plants expressing AtEFR driven by the constitutive rice actin promoter and tested their response to elf18. We show that transgenic expression of AtEFR in wheat confers recognition of elf18, as measured by the induction of immune marker genes and callose deposition. When challenged with the cereal bacterial pathogen Pseudomonas syringae pv.oryzae, transgenic EFR wheat lines had reduced lesion size and bacterial multiplication. These results demonstrate that AtEFR can be transferred successfully from dicot to monocot species, further revealing that immune signalling pathways are conserved across these distant phyla. As novel PRRs are identified, their transfer between plant families represents a useful strategy for enhancing resistance to pathogens in crops.