Haemaphysalis longicornis:: Molecular characterization of a homologue of the macrophage migration inhibitory factor from the partially fed ticks
Haemaphysalis longicornis:: Molecular characterization of a homologue of the macrophage migration inhibitory factor from the partially fed ticks
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DOI：
10.1016/j.exppara.2006.07.006
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发表时间：
2007-02-01
影响因子：
2.1
通讯作者：
Fujisaki, Kozo
中科院分区：
文献类型：
--
作者：
Umemiya, Rika;Hatta, Takeshi;Fujisaki, Kozo
巨噬细胞移动抑制因子(MIF)已在一些脊椎动物和无脊椎动物中被发现。MIF与脊椎动物的炎症、肿瘤生长和血管生成有关。在这里，我们报告了来自部分饲养的长角血蜱的MIF同源物的分子特征。对H1MIT基因的序列分析表明，其推导的氨基酸序列与美洲扁虱MIF基因的同源性为77%。用抗His-H1MIF抗体进行Western印迹分析表明，H1MIF在吸血过程中表达上调。免疫组织化学结果显示，部分摄食硬蜱的内源性H1MIF定位于中肠和表皮细胞。此外，功能实验表明，GST-H1MIF对人单核细胞的迁移有抑制作用。综上所述，我们认为H1MIF可能通过抑制宿主巨噬细胞向摄食部位的迁移而促进血液摄食，或者可能参与硬体细胞的增殖和分化。(C)2006 Elsevier Inc.保留所有权利。
The macrophage migration inhibitory factor (MIF) has been identified from some vertebrates and invertebrates. MIF is related to inflammation, tumor growth, and angiogenesis in vertebrates. Here, we report the molecular characterization of a homologue of MIF from partially fed Haemaphysalis, longicornis. The sequence analysis of the H. longicornis MIF (H1MIT) indicated that its deduced amino acid sequence has an identity of 77% with the MIF of the tick Amblyomma americanum. Western blot analysis using the anti-His-H1MIF antibody showed that H1MIF was up-regulated during blood feeding. Immunohistochemistry showed that the endogenous H1MIF in partially fed ticks was localized to the midgut and epidermal cells. Moreover, the functional assay revealed that the GST-H1MIF inhibited the migration of human monocytes. In conclusion, we consider that H1MIF may facilitate blood feeding by inhibiting host macrophage migration to the feeding lesion or may participate in the proliferation and differentiation of cells in the tick body. (c) 2006 Elsevier Inc. All rights reserved.