Proteomic patterns: their potential for disease diagnosis
Proteomic patterns: their potential for disease diagnosis
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DOI：
10.1016/j.mce.2004.10.010
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发表时间：
2005-01-31
影响因子：
4.1
通讯作者：
Issaq, HJ
中科院分区：
文献类型：
--
作者：
Xiao, Z;Prieto, D;Issaq, HJ
蛋白质丰度、结构或功能的改变在临床症状发展之前充当病理异常的有用指标，并且因此通常是有用的诊断和预后生物标志物。然而，癌症等疾病的潜在机制相当复杂，因为通常涉及多种失调的蛋白质。正是由于这个原因，最近的假设表明，检测生物标志物的面板可以提供更高的灵敏度和特异性的疾病诊断比提供与单一标志物。最近，出现了一种基于蛋白质模式分析的新方法，该方法可能提供更有效的方法来诊断疾病，如卵巢癌和前列腺癌。该方法基于使用表面增强激光解吸/电离（SELDI）飞行时间质谱（TOF-MS）来检测来自临床样品（例如血清和血浆）的差异捕获蛋白。这种分析导致在最近的研究中已经显示的“蛋白质组”模式的检测，以区分患病和未受影响的受试者在不同程度上。本文将讨论SELDI蛋白芯片技术的基础知识，并重点介绍其在疾病生物标志物发现，重点是癌症诊断的最新应用。(C)2004爱思唯尔爱尔兰有限公司保留所有权利。
Alterations in proteins abundance, structure, or function, act as useful indicators of pathological abnormalities prior to development of clinical symptoms and as such are often useful diagnostic and prognostic biomarkers. The underlying mechanism of diseases such as cancer are, however, quite complicated in that often multiple dysregulated proteins are involved. It is for this reason that recent hypotheses suggest that detection of panels of biomarkers may provide higher sensitivities and specificities for disease diagnosis than is afforded with single markers. Recently, a novel approach based on the analysis of protein patterns has emerged that may provide a more effective means to diagnose diseases, such as ovarian and prostate cancer. The method is based on the use of surface-enhanced laser desorption/ionization (SELDI) time-of-flight mass spectrometry (TOF-MS) to detect differentially captured proteins from clinical samples, such as serum and plasma. This analysis results in the detection of "proteomic" patterns that have been shown in recent investigations to distinguish diseased and unaffected subjects to varying degrees. This review will discuss the basics of SELDI protein chip technology and highlight its recent applications in disease biomarker discovery with emphasis on cancer diagnosis. (C) 2004 Elsevier Ireland Ltd. All rights reserved.