Embryo Retrieval after Hormonal Treatment to Control Ovarian Function and Non-surgical Artificial Insemination in African Lions (Panthera leo)
Embryo Retrieval after Hormonal Treatment to Control Ovarian Function and Non-surgical Artificial Insemination in African Lions (Panthera leo)
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DOI：
10.1111/rda.12026
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发表时间：
2012-12-01
影响因子：
1.7
通讯作者：
Hildebrandt, T. B.
中科院分区：
文献类型：
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作者：
Goeritz, F.;Painer, J.;Hildebrandt, T. B.
目录辅助生殖技术是繁殖濒危野生猫科动物的必要条件。人工授精（AI）已经在几个野生猫科动物物种中报道，但怀孕率很低，部分原因是目前的激素刺激方案失败。因此，本研究描述了应用可靠的方法来监测卵巢活动和开发一种有效的激素方案，以诱导非洲狮子发情和排卵。猪FSH和猪LH的应用被证明是有效的诱导卵泡生长和排卵，这种方案似乎上级先前描述的方案在排卵率和受精率。此外，在狮子中成功地进行了非手术AI，并收集子宫期胚胎并冷冻保存。非洲狮可以作为一个有价值的模式，以发展辅助生殖繁殖的文物动物园人口在极度濒危的亚洲狮或巴巴利狮。
Contents Assisted reproduction technologies are essential for propagating endangered wild felids. Artificial insemination (AI) has been reported in several wild feline species, but pregnancy rates are low, partially owing to failures of current hormonal stimulation protocols. Therefore, this study describes the application of reliable methods to monitor ovarian activity and the development of an effective hormonal protocol to induce oestrus and ovulation in African lions. Application of porcine FSH and porcine LH was shown to be effective for inducing follicular growth and ovulation, and this regimen appeared to be superior to protocols described earlier in terms of ovulation and fertilization rates. Furthermore, non-surgical AI was performed successfully in lions, and uterine-stage embryos were collected and cryopreserved. African lions may serve as a valuable model to develop assisted reproduction for propagation of relic zoo populations in the critically endangered Asian lion or Barbary lion.