Acetone-Butanol-Ethanol Production from Waste Seaweed Collected from Gwangalli Beach, Busan, Korea, Based on pH-Controlled and Sequential Fermentation Using Two Strains
Acetone-Butanol-Ethanol Production from Waste Seaweed Collected from Gwangalli Beach, Busan, Korea, Based on pH-Controlled and Sequential Fermentation Using Two Strains
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DOI：
10.1007/s12010-018-2711-9
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发表时间：
2018-08-01
影响因子：
3
通讯作者：
Kim, Sung-Koo
中科院分区：
文献类型：
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作者：
Sunwoo, In Yung;Nguyen Trung Hau;Kim, Sung-Koo
以韩国釜山光加利海滩废弃海藻为原料，研究了丙酮-丁醇-乙醇（ABE）的最佳生产条件。废弃海藻中含有纤维和碳水化合物，含量为48.34%，是生产ABE的主要资源。基于超热（HT）酸水解废海藻获得单糖的最佳条件是浆料含量为8%，硫酸浓度为138 mM，处理时间为10 min。酶糖化进行使用16单位/mL的Viscozyme L，表现出最高的亲和力（K（m）= 1.81 g/L）。经预处理后，得到34.0g/L的单糖。ABE发酵用丙酮丁醇梭菌和酪丁酸梭菌的单一和顺序发酵进行;这控制pH。使用C. tyrobutyricum和C.丙酮丁醇使用pH控制的培养液和连续细胞培养从废弃海藻中高效生产ABE。
The optimal conditions for acetone-butanol-ethanol (ABE) production were evaluated using waste seaweed from Gwangalli Beach, Busan, Korea. The waste seaweed had a fiber and carbohydrate, content of 48.34%; these are the main resources for ABE production. The optimal conditions for obtaining monosaccharides based on hyper thermal (HT) acid hydrolysis of waste seaweed were slurry contents of 8%, sulfuric acid concentration of 138 mM, and treatment time of 10 min. Enzymatic saccharification was performed using 16 unit/mL Viscozyme L, which showed the highest affinity (K (m) = 1.81 g/L). After pretreatment, 34.0 g/L monosaccharides were obtained. ABE fermentation was performed with single and sequential fermentation of Clostridium acetobutylicum and Clostridium tyrobutyricum; this was controlled for pH. A maximum ABE concentration of 12.5 g/L with Y (ABE) 0.37 was achieved using sequential fermentation with C. tyrobutyricum and C. acetobutylicum. Efficient ABE production from waste seaweed performed using pH-controlled culture broth and sequential cell culture.