Behavioral response of the malaria mosquito, Anopheles gambiae, to human sweat inoculated with axilla bacteria and to volatiles composing human axillary odor
Behavioral response of the malaria mosquito, Anopheles gambiae, to human sweat inoculated with axilla bacteria and to volatiles composing human axillary odor
复制标题
DOI：
10.1093/chemse/bjw106
复制
发表时间：
2017-02-01
期刊：
影响因子：
3.5
通讯作者：
Guerin, Patrick M.
中科院分区：
文献类型：
--
作者：
Frei, Jerome;Krober, Thomas;Guerin, Patrick M.
在双选择嗅觉计中记录了冈比亚按蚊（双翅目：蚊科）对与人类腋窝细菌一起培养的雄性和雌性腋窝汗液气味的反应。选择表皮葡萄球菌是因为其低气味产生模式，选择杰氏棒状杆菌是因为其具有与酰基谷氨酰胺氨酰化酶活性成比例的强N，可释放羧酸，包括(R)/(S)-3-羟基-3-甲基己酸(HMHA)，选择溶血葡萄球菌是因为其能够释放含硫化合物，包括(R/S)-3-甲基3- 1-硫烷基己醇 (MSH)。冈比亚按蚊的行为反应根据（i）其起飞和持续逆风飞行的反应性和（ii）其一只或两只手臂上带有测试气味的两个嗅觉计臂之间的辨别能力进行评估。实验是在二氧化碳脉冲作为行为敏化剂存在的情况下进行的。冈比亚按蚊通过嗅觉计臂清晰地区分了通过用雄性或雌性汗液孵育三种细菌中的任何一种而产生的气味。而安.冈比亚蚊子在嗅觉计中不区分雄性和雌性无菌汗液样本，与用作接种物的物种无关，与使用相同细菌孵育的雌性汗液相比，蚊子始终表现出对雄性汗液的偏好。与吉克棒杆菌一起孵育的汗液使蚊子特别敏感，这种底物引起了对雄性汗液的最强烈偏好，而不是对雌性汗液的偏好。经过单独测试，HMHA 和 MSH 均未引起明显的辨别反应，但确实影响了蚊子的反应性。这些发现为进一步研究按蚊气味介导的亲人宿主寻找行为奠定了基础。冈比亚。
The responses of Anopheles gambiae Giles sensu stricto (Diptera: Culicidae) to odors from male and female axillary sweat incubated with human axilla bacteria were recorded in a dualchoice olfactometer. Staphylococcus epidermidis was selected for its low odor- producing pattern, Corynebacterium jeikeium for its strong N proportional to-acylglutamine aminoacylase activity liberating carboxylic acids including (R)/(S)-3-hydroxy-3-methylhexanoic acid (HMHA) and Staphylococcus haemolyticus for its capacity to liberate sulfur- containing compounds including (R/S)-3-methyl3- sulfanylhexan-1-ol (MSH). Anopheles gambiae behavioral responses were evaluated under (i) its responsiveness to take off and undertake sustained upwind flight and (ii) its discriminating capacity between the two olfactometer arms bearing a test odor in either one or both arms. Experiments were conducted in the presence of carbon dioxide pulses as a behavioral sensitizer. Anopheles gambiae clearly discriminated for the olfactometer arm conveying odor generated by incubating any of the three bacteria species with either male or female sweat. Whereas An. gambiae did not discriminate between male and female sterile sweat samples in the olfactometer, the mosquito consistently showed a preference for male sweat over female sweat incubated with the same bacterium, independent of the species used as inoculum. Sweat incubated with C. jeikeium rendered mosquitoes particularly responsive and this substrate elicited the strongest preference for male over female sweat. Tested on their own, neither HMHA nor MSH elicited a clear discriminating response but did affect mosquito responsiveness. These findings serve as a basis for further research on the odor- mediated anthropophilic host-seeking behavior of An. gambiae.