Marine Bioluminescence with Dehydrocoelenterazine, an Imidazopyrazinone Compound.
Marine Bioluminescence with Dehydrocoelenterazine, an Imidazopyrazinone Compound.
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使用脱氢腔肠素（一种咪唑并吡嗪酮化合物）进行海洋生物发光。
DOI：
10.1007/7081_2020_41
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Masaki Kuse
中科院分区：
文献类型：
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作者：
Maiko Moriguchi;RyoTakahashi;Bubwoong Kang;Masaki Kuse;Masaki Kuse
杂环在生物发光中起着重要的作用。其中咪唑吡嗪酮类化合物在一些海洋生物发光系统中用于发光。Coelenterazine （CL）是水母蛋白的有机物，水母蛋白是一种光蛋白，具有绿色荧光蛋白（GFP）。氯化氯的脱氢氧化得到脱氢钴enterazine (DCL)，它也是一种海洋生物发光的有机物。DCL不能与氧直接反应。因此，它被转化为发色团，这是光蛋白发光所必需的。在活性氧（ROS）的存在下，发色团直接与氧发生反应，发出可见光。辛plectin是一种来自乌贼的光蛋白，而Pholasin是一种来自软体动物的光蛋白，它们利用dcl衍生的发色团。Pholasin是Knight Scientific Ltd.的注册商标，被广泛用作ROS的高灵敏度指标，即Pholasin检测活化的中性粒细胞产生的ROS是氧化应激的生物标志物。鉴于目前已有许多关于cl依赖性生物发光的优秀综述，本文主要介绍了以DCL为有机物质的生物发光系统的研究历史以及DCL衍生发色团的生物发光机制。
Heterocycles play important roles in bioluminescence. Among them, the imidazopyrazinone compounds are utilized for light emission in some marine bioluminescent systems. Coelenterazine (CL) is the organic substance of aequorin, a photoprotein obtained from jellyfish, that possesses green fluorescent proteins (GFP). The dehydrogenative oxidation of CL affords dehydrocoelenterazine (DCL), which is also an organic substance for marine bioluminescence. DCL cannot react directly with oxygen. Therefore, it is converted to the chromophore, which is required for light emission in a photoprotein. The chromophore directly reacts with oxygen in the presence of reactive oxygen species (ROS) to emit visible light. Symplectin, a photoprotein derived from squids, and Pholasin, a photoprotein of mollusks, utilize the DCL-derived chromophore. Pholasin, a registered trademark of Knight Scientific Ltd., is widely utilized as a highly sensitive indicator of ROS, i.e., the detection of ROS that are produced from activated neutrophils by Pholasin is the biomarker for oxidative stresses. Since there are many excellent reviews regarding CL-dependent bioluminescence, this review focuses on the bioluminescent systems, which employ DCL as an organic substance, by describing the history of the research on these systems and the bioluminescent mechanism that is related to the DCL-derived chromophores.