NUCLEAR AND MITOCHONDRIAL INHERITANCE IN YEAST DEPENDS ON NOVEL CYTOPLASMIC STRUCTURES DEFINED BY THE MDM1 PROTEIN
NUCLEAR AND MITOCHONDRIAL INHERITANCE IN YEAST DEPENDS ON NOVEL CYTOPLASMIC STRUCTURES DEFINED BY THE MDM1 PROTEIN
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DOI：
10.1083/jcb.118.2.385
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发表时间：
1992-07-01
影响因子：
7.8
通讯作者：
YAFFE, MP
中科院分区：
文献类型：
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作者：
MCCONNELL, SJ;YAFFE, MP
mdm 1突变导致酵母细胞在非允许温度下的温度敏感性生长和细胞核和线粒体向发育中的芽的缺陷转移。通过互补法克隆了MDM 1基因，其序列显示一个开放阅读框架，编码51.5 kD的潜在蛋白产物。这种蛋白质显示氨基酸序列相似性仓鼠波形蛋白和小鼠表皮角蛋白。基因破坏表明MDM 1是有丝分裂生长所必需的。针对MDM 1蛋白的抗体识别51-kD多肽，其通过间接免疫荧光定位于分布在整个酵母细胞质中的斑点和点状阵列的新模式。这些结构消失后，转移mdm 1突变细胞到非允许的温度，虽然细胞水平的MDM 1蛋白是不变的。通过动物细胞的间接免疫荧光，针对MDM 1的亲和纯化抗体也特异性地识别中间丝。这些结果表明，新的细胞质结构含有MDM 1蛋白介导的酵母细胞器遗传。
The mdm1 mutation causes temperature-sensitive growth and defective transfer of nuclei and mitochondria into developing buds of yeast cells at the nonpermissive temperature. The MDM1 gene was cloned by complementation, and its sequence revealed an open reading frame encoding a potential protein product of 51.5 kD. This protein displays amino acid sequence similarities to hamster vimentin and mouse epidermal keratin. Gene disruption demonstrated that MDM1 is essential for mitotic growth. Antibodies against the MDM1 protein recognized a 51-kD polypeptide that was localized by indirect immunofluorescence to a novel pattern of spots and punctate arrays distributed throughout the yeast cell cytoplasm. These structures disappeared after shifting mdm1 mutant cells to the nonpermissive temperature, although the cellular level of MDM1 protein was unchanged. Affinity-purified antibodies against MDM1 also specifically recognized intermediate filaments by indirect immunofluorescence of animal cells. These results suggest that novel cytoplasmic structures containing the MDM1 protein mediate organelle inheritance in yeast.