Polarity orientation of axonal microtubules.
Polarity orientation of axonal microtubules.
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DOI：
10.1083/jcb.91.3.661
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发表时间：
1981-12
期刊：
影响因子：
--
通讯作者：
Hamborg MA
中科院分区：
文献类型：
--
作者：
Heidemann SR;Landers JM;Hamborg MA
细胞微管的极性取向被广泛认为是理解微管组装和微管运动的控制在体内是重要的。我们使用了海德曼和麦金托什（自然（伦敦）的方法的修改。286：517-519）来确定猫的节后交感神经纤维中轴突微管的极性取向。在来自三只猫的纤维中，我们能够可视化68%的轴突微管的极性;其中，96%显示出相同的极性取向。我们的解释是，所有轴突微管的快速生长的末端都在细胞体的远端。我们支持Kirschner关于微管组织中心的假说。(J. Cell Biol.86：330- 334），尽管这种解释提出了关于轴突微管连续性的问题。我们的研究结果是不一致的轴突运输模型的基础上的力的方向是由微管的极性决定的微管表面上的生产。
The polarity orientation of cellular microtubules is widely regarded to be important in understanding the control of microtubule assembly and microtubule-based motility in vivo. We have used a modification of the method of Heidemann and McIntosh (Nature (Lond.). 286:517-519) to determine the polarity orientation of axonal microtubules in postganglionic sympathetic fibers of the cat. In fibers from three cats we were able to visualize the polarity of 68% of the axonal microtubules; of these, 96% showed the same polarity orientation. Our interpretation is that the rapidly growing end of all axonal microtubules is distal to the cell body. We support Kirschner's hypothesis on microtubule organizing centers. (J. Cell Biol. 86:330- 334), although this interpretation raises questions about the continuity of axonal microtubules. Our results are inconsistent with a number of models for axonal transport based on force production on the surface of microtubules in which the direction of force is determined by the polarity of microtubules.