Response of Bacillus cereus spores to high hydrostatic pressure and moderate heat
Response of Bacillus cereus spores to high hydrostatic pressure and moderate heat
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DOI：
10.1016/j.lwt.2007.11.011
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发表时间：
2008-12
期刊：
影响因子：
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通讯作者：
Xingrong Ju;Yu-long Gao;Minglan Yao;Y. Qian
中科院分区：
文献类型：
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作者：
Xingrong Ju;Yu-long Gao;Minglan Yao;Y. Qian
研究了高压（400- 600 MPa）和中热（60-80°C）在不同处理时间（10- 20 min）处理对悬浮在牛奶缓冲液中的蜡状芽孢杆菌As 1.1846孢子的还原作用。采用响应面法建立了高静水压灭活的二次方程。通过分析响应面图和相应的等值线图，并通过求解二次方程，实验值与预测值显着一致，因为调整后的决定系数（RSD 2）为0.9752，显著性水平为P<0.0001。最佳工艺参数可使B的循环减少6个对数。蜡状芽孢的最佳工艺条件为：压力540.0MPa，温度71 ℃，保压时间16.8min。充分的模型方程在预测最佳响应值进行了有效地验证，使用实验测试数据中没有使用的模型的发展。
The effects of high pressure (400–600MPa) and moderate heat (60–80°C) treatments at various process times (10–20min) on the reduction of Bacillus cereus As 1.1846 spores, suspended in milk buffer were investigated. In the present work, response surface methodology (RSM) was employed, and a quadratic equation of high hydrostatic pressure inactivation was built with RSM. By analyzing response surface plots and corresponding contour plots and by solving the quadratic equation, experimental values were shown to be significantly in agreement with predicted values, since the adjusted determination coefficient (RAdj2) was 0.9752 and the level of significance was P<0.0001. Optimum process parameters for a six-log cycle reduction of B. cereus spores were obtained: pressure, 540.0MPa; temperature, 71°C; and pressure-holding time, 16.8min. The adequacy of the model equation in predicting optimum response values was verified effectively using experimental test data that was not used in the development of the model.