Localization of glucokinase-like immunoreactivity in the rat lower brain stem: For possible location of brain glucose-sensing mechanisms
Localization of glucokinase-like immunoreactivity in the rat lower brain stem: For possible location of brain glucose-sensing mechanisms
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DOI：
10.1210/en.141.1.375
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发表时间：
2000-01-01
期刊：
影响因子：
4.8
通讯作者：
Maeda, K
中科院分区：
文献类型：
--
作者：
Maekawa, F;Toyoda, Y;Maeda, K
胰腺葡萄糖激酶（GK）被认为是胰腺P细胞葡萄糖敏感单位的重要组成部分。这是可能的，大脑使用类似的葡萄糖传感单元，我们采用GK免疫组织化学和共聚焦显微镜检查GK样免疫反应在大鼠大脑的解剖分布。我们发现强烈的GK样免疫反应室管膜细胞，内皮细胞，和许多肾上腺素能神经元。在室管膜细胞中，GK样免疫反应位于胞浆区，而不在细胞核中。GK阳性室管膜细胞纤毛上有葡萄糖转运蛋白2（GLUT 2）样免疫反应。此外，室管膜细胞的纤毛上有GLUT 1样免疫反应性，GLUT 4样免疫反应性密集于胞质区，而质膜上有少量。在肾上腺素能神经元中，GK样免疫反应见于胞质及其突起。目前的研究结果提出了这样一种可能性，即这些GK样免疫阳性细胞构成了大脑中巨大的葡萄糖传感机制的一部分。
Pancreatic glucokinase (GK) is considered an important element of the glucose-sensing unit in pancreatic P-cells. It is possible that the brain uses similar glucose-sensing units, and we employed GK immunohistochemistry and confocal microscopy to examine the anatomical distribution of GK-like immunoreactivities in the rat brain. We found strong GK-like immunoreactivities in the ependymocytes, endothelial cells, and many serotonergic neurons. In the ependymocytes, the GK-like immunoreactivity was located in the cytoplasmic area, but not in the nucleus. The GK-positive ependymocytes were found to have glucose transporter-2 (GLUT2)-like immunoreactivities on the cilia. In addition, the ependymocytes had GLUT1-like immunoreactivity on the cilia and GLUT4-like immunoreactivity densely in the cytoplasmic area and slightly in the plasma membrane. In serotonergic neurons, GK-like immunoreactivity was found in the cytoplasm and their processes. The present results raise the possibility that these GK-like immunopositive cells comprise a part of a vast glucose-sensing mechanism in the brain.