Large Differences in Gene Expression Responses to Drought and Heat Stress between Elite Barley Cultivar Scarlett and a Spanish Landrace.
Large Differences in Gene Expression Responses to Drought and Heat Stress between Elite Barley Cultivar Scarlett and a Spanish Landrace.
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DOI：
10.3389/fpls.2017.00647
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发表时间：
2017
影响因子：
5.6
通讯作者：
Contreras-Moreira B
中科院分区：
文献类型：
--
作者：
Cantalapiedra CP;García-Pereira MJ;Gracia MP;Igartua E;Casas AM;Contreras-Moreira B
干旱每个季节都给农作物生产造成重大损失。耐旱性的改进可以利用种质资源的多样性，其中大部分还没有被纳入现代育种。西班牙地方品种构成了一个有前途的资源大麦育种，因为他们被广泛种植，直到上个世纪，仍然显示出良好的生产能力，在压力下。在这里，我们研究了两种基因型的转录组表达景观，一个优秀的西班牙地方品种衍生的近交系（SBCC073）和现代品种（斯嘉丽）。成年植物的基因表达后，长期的压力，无论是干旱或干旱与热相结合，进行了监测。在重度干旱条件下，成熟叶片的转录组变化不大，而在轻度干旱和高温复合条件下，成熟叶片的基因表达发生了丰富的变化。SBCC073的花序发育过程中基因表达基本保持不变，而Scarlett的花序发育过程中基因表达发生了大量变化。生理性状和基因表达谱的基因型差异证实了地方品种SBCC 073和栽培品种Scarlett在非生物胁迫下的不同行为，表明它们采取不同的胁迫响应策略。与大麦的相关研究比较，解决基因表达对干旱的反应，揭示了共同的生物过程，但关于个人差异表达的转录本的协议温和。特别强调的是在寻找共表达的基因和潜在的共同调控基序。总的来说，11个转录因子被确定，其中之一匹配的顺式调控基序发现上游的共表达基因参与这些反应。
Drought causes important losses in crop production every season. Improvement for drought tolerance could take advantage of the diversity held in germplasm collections, much of which has not been incorporated yet into modern breeding. Spanish landraces constitute a promising resource for barley breeding, as they were widely grown until last century and still show good yielding ability under stress. Here, we study the transcriptome expression landscape in two genotypes, an outstanding Spanish landrace-derived inbred line (SBCC073) and a modern cultivar (Scarlett). Gene expression of adult plants after prolonged stresses, either drought or drought combined with heat, was monitored. Transcriptome of mature leaves presented little changes under severe drought, whereas abundant gene expression changes were observed under combined mild drought and heat. Developing inflorescences of SBCC073 exhibited mostly unaltered gene expression, whereas numerous changes were found in the same tissues for Scarlett. Genotypic differences in physiological traits and gene expression patterns confirmed the different behavior of landrace SBCC073 and cultivar Scarlett under abiotic stress, suggesting that they responded to stress following different strategies. A comparison with related studies in barley, addressing gene expression responses to drought, revealed common biological processes, but moderate agreement regarding individual differentially expressed transcripts. Special emphasis was put in the search of co-expressed genes and underlying common regulatory motifs. Overall, 11 transcription factors were identified, and one of them matched cis-regulatory motifs discovered upstream of co-expressed genes involved in those responses.