Reactive oxygen species are involved in lysophosphatidic acid-induced apoptosis in rat cerebellar granule cells
Reactive oxygen species are involved in lysophosphatidic acid-induced apoptosis in rat cerebellar granule cells
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DOI：
10.1163/156856702760129500
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发表时间：
2002-02
影响因子：
3.3
通讯作者：
Zhaohui Zhang;T. Wei;J. Hou;Gengshan Li;Shaozu Yu;W. Xin
中科院分区：
文献类型：
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作者：
Zhaohui Zhang;T. Wei;J. Hou;Gengshan Li;Shaozu Yu;W. Xin
溶血磷脂酸（LPA）诱导原代大鼠小脑颗粒细胞凋亡，其形态学特征为染色质浓缩和凋亡小体形成。以DCFH-DA和DHR 123为荧光探针，采用激光共聚焦扫描显微镜（LCSM）观察细胞凋亡过程中内源性活性氧（ROS）的形成。抗氧化剂川芎嗪（TMP）预处理可有效抑制内源性ROS的产生，保护神经元免于凋亡。结果提示，ROS可能参与了LPA诱导的神经元凋亡。
Lysophosphatidic acid (LPA) induced apoptosis in primary rat cerebellar granule cells, which was characterized morphologically by chromatin condensation and the formation of apoptotic bodies. With redox-sensitive fluorescence probes DCFH-DA and DHR123, the formation of endogenous reactive oxygen species (ROS) inside cells during the apoptosis process was monitored by laser confocal scanning microscopy (LCSM). Pretreatment with the antioxidant tetramethylpyrazine (TMP) could effectively inhibit the formation of endogenous ROS and protect neurons from apoptosis. The results suggest that ROS might be involved in LPA-induced apoptosis in neurons.