Ancestry and diversity of BEL1-like homeobox genes revealed by gymnosperm (Gnetum gnemon) homologs
Ancestry and diversity of BEL1-like homeobox genes revealed by gymnosperm (Gnetum gnemon) homologs
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DOI：
10.1007/s00427-002-0259-7
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发表时间：
2002-10-01
影响因子：
2.4
通讯作者：
Theissen, G
中科院分区：
文献类型：
--
作者：
Becker, A;Bey, M;Theissen, G
bell样同源盒基因编码植物特异性转录因子，其中至少有一些对胚珠发育很重要。在这里，我们报道了MELBEL1-MELBEL4，这是第一个来自非开花植物裸子植物葵属植物的条带状基因。我们的分析表明，大约3亿年前，在现存种子植物最近的共同祖先中，已经至少存在一个bell样基因。多个序列比对显示，从那时起，不仅dna结合同源结构域，而且同源结构域上游的蛋白-蛋白相互作用结构域BEL结构域也高度保守。BEL结构域与其他TALE同源结构域蛋白（MEIS、KNOX和PBC）中保守的上游结构域的序列比较显示，其序列相似性较弱。然而，由于MEIS、KNOX和PBC结构域具有同源性，并且KNOX和BEL结构域在体内直接相互作用，BEL结构域很可能也来源于一个祖先上游（MEINOX）结构域。如果您在http://dx.doi.org/10.1007/s00427-002-0259-7访问本文，可以获得电子补充材料。在那个页面上（左边的框架），有一个链接直接把你带到补充材料。
BELL-like homeobox genes encode plant-specific transcription factors, at least some of which are important for ovule development. Here we report MELBEL1-MELBEL4, the first BELT-like genes from a non-flowering plant, the gymnosperm Gnetum gnemon. Our analyses suggest that there was already at least one BELL-like gene present in the most recent common ancestor of extant seed plants about 300 million years ago. Multiple sequence alignments revealed that since this time, not only the DNA-binding homeodomain, but also a protein-protein interaction domain upstream of the homeodomain, termed the BEL domain, has been highly conserved. Sequence comparison of the BEL domain with upstream domains that have been conserved in other TALE homeodomain proteins, i.e. MEIS, KNOX, and PBC, revealed only weak sequence similarity. However, since homology has been shown for MEIS, KNOX, and PBC domains and since KNOX and BEL domains directly interact in vivo, it appears likely that the BEL domain was also derived from an ancestral upstream (MEINOX) domain. Electronic Supplementary Material is available if you access this article at http://dx.doi.org/10.1007/s00427-002-0259-7. On that page (frame on the left side), a link takes you directly to the supplementary material.