PURIFICATION OF THE CYTOCHALASIN-B BINDING-COMPONENT OF THE HUMAN-ERYTHROCYTE MONOSACCHARIDE TRANSPORT-SYSTEM
PURIFICATION OF THE CYTOCHALASIN-B BINDING-COMPONENT OF THE HUMAN-ERYTHROCYTE MONOSACCHARIDE TRANSPORT-SYSTEM
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DOI：
10.1016/0005-2736(79)90257-8
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发表时间：
1979-01-01
期刊：
影响因子：
--
通讯作者：
LIENHARD, GE
中科院分区：
文献类型：
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作者：
BALDWIN, SA;BALDWIN, JM;LIENHARD, GE
纯化了人红细胞单糖转运系统的细胞松弛素B结合组分。该制剂似乎含有1种主要蛋白质，其表观多肽链MW为55，000，每条链约有0.4个结合位点。细胞松弛素B以1.3 cnt的Kd与重构的制剂结合。10-7 M，该值与完整红细胞中转运系统报告的值相似。
The cytochalasin B binding component of the human erythrocyte monosaccharide transport system was purified. The preparation appeared to contain 1 major protein with an apparent polypeptide chain MW of 55,000 and about 0.4 binding sites per chain. Cytochalasin B bound to the reconstituted preparation with a Kd of 1.3 .cntdot. 10-7 M, a value which is similar to that reported for the transport system in the intact erythrocyte.