HUMAN ALVEOLAR MACROPHAGE GROWTH-FACTOR FOR FIBROBLASTS - REGULATION AND PARTIAL CHARACTERIZATION
HUMAN ALVEOLAR MACROPHAGE GROWTH-FACTOR FOR FIBROBLASTS - REGULATION AND PARTIAL CHARACTERIZATION
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DOI：
10.1172/jci110677
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发表时间：
1982-01-01
影响因子：
15.9
通讯作者：
CRYSTAL, RG
中科院分区：
文献类型：
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作者：
BITTERMAN, PB;RENNARD, SI;CRYSTAL, RG
通过严格调节成纤维细胞复制速率，将构成肺泡结构的成纤维细胞数量控制在狭窄的范围内。肺泡成纤维细胞生长调节信号的一个可能来源是肺泡巨噬细胞，它是单核吞噬细胞家族的一员，众所周知，它们是多种靶细胞生长因子的重要来源。为了评估肺泡巨噬细胞在控制肺泡成纤维细胞复制中的作用，通过支气管肺泡灌洗获得的正常个体的巨噬细胞在添加和不添加刺激的情况下维持悬浮培养，并评估上清液的成纤维细胞生长促进作用。来自未刺激巨噬细胞的上清液不含生长因子活性。用颗粒和免疫复合物刺激的巨噬细胞的上清液含有一种生长因子，可导致成纤维细胞复制率显着增加。巨噬细胞刺激后3-4小时观察到最大生长因子活性，浓度为1-2倍。 106 个巨噬细胞/ml。在中性 pH 值、0.27 M NaCl 条件下从 DEAE 纤维素中洗脱的肺泡巨噬细胞衍生生长因子的表观分子量为 18,000，并且似乎与其他表征的生长因子不同。肺泡巨噬细胞衍生的生长因子在 12 小时内刺激肺成纤维细胞 DNA 合成，细胞分裂在 48 小时内明显。在无血清培养中，肺泡巨噬细胞衍生生长因子本身并不促进成纤维细胞复制，而是作为进展因子，在成纤维细胞生长因子或血小板衍生生长因子等能力因子存在下导致成纤维细胞复制率协同增加。当受到刺激时，人肺泡巨噬细胞可以通过在肺泡宏观环境中释放生长因子来部分调节肺泡成纤维细胞的复制率。
The number of fibroblasts composing the alveolar structures is controlled within narrow limits by a strictly modulated rate of fibroblast replication. One possible source of growth-modulating signals for alveolar fibroblasts is the alevolar macrophage, a member of the mononuclear phagocyte family of cells, which collectively are known to be important sources of growth factors for a variety of target cells. To evaluate the role of alveolar macrophages in the control of alveolar fibroblast replication, macrophages from normal individuals obtained by bronchoalveolar lavage were maintained in suspension culture with and without added stimuli, and supernates were evaluated for fibroblast growth-promoting effect. Supernates from unstimulated macrophages contained no growth factor activity. Supernates from macrophages stimulated with particulates and immune complexes contained a growth factor that caused a significant increase in fibroblast replication rate. Maximum growth factor activity was observed 3-4 h after macrophage stimulation, at a concentration of 1-2 .times. 106 macrophages/ml. The alveolar macrophage-derived growth factor eluted from DEAE-cellulose at 0.27 M NaCl at neutral pH had an apparent MW of 18,000 and appeared to be distinct from other characterized growth factors. The alveolar macrophage-derived growth factor stimulated lung fibroblast DNA synthesis within 12 h, with cell division apparent within 48 h. In serum-free culture, the alveolar macrophage-derived growth factor by itself did not promote fibroblast replication, but acted as a progression factor causing a synergistic increase in fibroblast replication rate in the presence of competence factors such as fibroblast growth factor or platelet-derived growth factor. When stimulated, human alveolar macrophages may modulate, in part, the replication rate of alveolar fibroblasts by releasing a growth factor within the alveolar macroenvironment.