Maternal residential proximity to major roadways, birth weight, and placental DNA methylation.
Maternal residential proximity to major roadways, birth weight, and placental DNA methylation.
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DOI：
10.1016/j.envint.2016.03.020
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发表时间：
2016-07
影响因子：
11.8
通讯作者：
Wellenius GA
中科院分区：
文献类型：
--
作者：
Kingsley SL;Eliot MN;Whitsel EA;Huang YT;Kelsey KT;Marsit CJ;Wellenius GA
胎儿发育期间暴露于交通污染与胎儿生长减少有关，有证据表明，胎盘中以变异DNA甲基化形式存在的表观遗传机制可能是这种影响的潜在机制。研究住宅区与最近的主要道路之间的关系，作为交通相关污染，胎儿生长和胎盘DNA甲基化的标志。我们从471名分娩后的妇女中获得了居住地址、胎盘样本和人口统计学数据。使用广义线性模型，我们评估了居住在主要道路附近（定义为居住在距离主要公路或主要道路≤150 m或距离次要道路≤50 m的地方）与胎儿生长和重复元件（LINE-1和Yb 8）的DNA甲基化之间的关联。我们评估了215名女性的表观基因组范围内的甲基化，以进一步研究与主要道路邻近度相关的DNA甲基化的特定变异。居住在主干道附近与175.9 g的（95% CI：-319.4，-32.5; p=0.016）出生体重较低，1.8（95% CI：0.9，3.8; p=0.09）乘以小于胎龄儿的几率，（95% CI：−1.57，−0.07; p=0.03）在完全校正的模型中，平均胎盘LINE-1甲基化水平较低。在表观基因组分析中，7个CpG位点与主要道路的住宅接近程度显著相关。胎盘甲基化的额外调整并没有减弱道路接近度和出生体重之间的关联。居住在主要道路附近与胎儿生长较低和显著的胎盘表观遗传变化有关。然而，所观察到的表观遗传变化似乎不足以解释所观察到的道路接近和胎儿生长之间的关联。
Exposure to traffic pollution during fetal development has been associated with reduced fetal growth, and there is evidence to suggest that epigenetic mechanisms in the placenta in the form of variant DNA methylation may be a potential mechanism of this effect. To examine the association between residential proximity to nearest major roadway, as a marker of traffic-related pollution, fetal growth and placental DNA methylation. We obtained residential addresses, placenta samples, and demographic data from 471 women following delivery. Using generalized linear models we evaluated the association between living close to a major roadway (defined as living ≤150 m from a primary highway or primary road or ≤50 m from a secondary road) and fetal growth and DNA methylation of repetitive elements (LINE-1 and AluYb8). We evaluated epigenome-wide methylation in a subset of 215 women to further investigate specific variation in DNA methylation associated with proximity to major roadways. Living close to a major roadway was associated with a 175.9 g (95% CI: -319.4, -32.5; p=0.016) lower birth weight, 1.8 (95% CI: 0.9, 3.8; p=0.09) times the odds of being small for gestational age, and 0.82 percentage points (95% CI: −1.57, −0.07; p=0.03) lower mean placental LINE-1 methylation levels in fully adjusted models. In epigenome-wide analyses, 7 CpG sites were significantly associated with residential proximity to major roadways. Additional adjustment for placental methylation did not attenuate the association between roadway proximity and birth weight. Living close to major roadways was associated with lower fetal growth and significant placental epigenetic changes. However, the observed epigenetic changes appear insufficient to explain the observed association between roadway proximity and fetal growth.