Determination of the levels of tryptophan and 12 metabolites in milk by liquid chromatography-tandem mass spectrometry with the QuEChERS method
Determination of the levels of tryptophan and 12 metabolites in milk by liquid chromatography-tandem mass spectrometry with the QuEChERS method
复制标题
QuEChERS 法液相色谱-串联质谱法测定牛奶中色氨酸和 12 种代谢物的含量
DOI：
10.3168/jds.2020-18260
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发表时间：
2020-11-01
影响因子：
3.5
通讯作者：
Chen, Gang
中科院分区：
文献类型：
--
作者：
Su, Meicheng;Cheng, Yongyou;Chen, Gang
色氨酸及其代谢物在人类中具有重要的生物学功能。牛奶是色氨酸摄入的重要来源。在这项研究中，我们开发了一种检测牛奶中色氨酸和 12 种代谢物水平的方法。使用 QuEChERS（快速、简单、廉价、有效、坚固且安全）程序提取分析物，并通过液相色谱-串联质谱法和电喷雾电离进行分析。所提出的方法产生了合适的精度（标准差
Tryptophan and metabolites have important biological functions in humans. Milk is an important source of tryptophan intake. In this study, we developed a method to detect levels of tryptophan and 12 metabolites in milk. The analytes were extracted by using the QuEChERS (quick, easy, cheap, effective, rugged, and safe) procedure and analyzed by liquid chromatography-tandem mass spectrometry with electrospray ionization. The proposed method resulted in suitable accuracy (standard deviation