Urokinase production by embryonic endocardial-derived cells: regulation by substrate composition.
Urokinase production by embryonic endocardial-derived cells: regulation by substrate composition.
复制标题
胚胎心内膜衍生细胞产生尿激酶：底物成分的调节。
DOI：
10.1006/dbio.1993.1042
复制
发表时间：
1993
影响因子：
2.7
通讯作者：
Alexander,SM
中科院分区：
文献类型：
--
作者：
McGuire,PG;Alexander,SM
心血管形态发生的早期事件以细胞迁移和广泛的组织重塑为特征。我们感兴趣的是细胞外丝氨酸蛋白酶尿激酶在这些事件中发挥的作用。尿激酶活性和mRNA水平升高已被证明与鹌鹑胚胎房室管和流出道的内膜垫组织中心室小梁形成和间充质细胞迁移的发生相关。在这项研究中，尿激酶生产的分离心内膜来源的细胞被发现受到影响的组成的基质中的细胞被暴露。细胞与45 kDa的明胶结合片段的纤连蛋白的相互作用上调尿激酶的生产近三倍。尿激酶活性的增加对细胞运动和扩散有深远的影响。
Early events in cardiovascular morphogenesis are characterized by cell migrations and extensive tissue remodeling. We are interested in the role played by the extracellular serine protease urokinase in these events. Elevated urokinase activity and mRNA levels have been shown to be associated with the onset of ventricular trabeculation and mesenchymal cell migration in the endocardial cushion tissues of the artioventricular canal and the outflow tract of the quail embryo. In this study, urokinase production by isolated endocardial-derived cells was found to be affected by the composition of the matrix to which the cells were exposed. Interaction of cells with a 45-kDa gelatin-binding fragment of fibronectin upregulated the production of urokinase by nearly threefold. This increase in urokinase activity had profound influences on cell motility and spreading.