Sequenced response of extracellular matrix deadhesion and fibrotic regulators after muscle damage is involved in protection against future injury in human skeletal muscle
Sequenced response of extracellular matrix deadhesion and fibrotic regulators after muscle damage is involved in protection against future injury in human skeletal muscle
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DOI：
10.1096/fj.10-176487
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发表时间：
2011-06-01
期刊：
影响因子：
4.8
通讯作者：
Langberg, Henning
中科院分区：
文献类型：
--
作者：
Mackey, Abigail L.;Brandstetter, Simon;Langberg, Henning
本研究的目的是验证骨骼肌细胞外基质（ECM）重塑参与保护人体肌肉免受损伤的假设。在单次电刺激(B)或30天后的重复电刺激（RB）或单次电刺激（RBc）后30 d，对内侧腓肠肌进行活检。从对照组腿上采集肌肉活检，与受刺激的腿进行比较。免疫组织化学检测卫星细胞含量、腱素C和肌肉再生；实时荧光定量PCR检测胶原蛋白、层粘胶蛋白、热休克蛋白（HSPs）、炎症及相关生长因子mRNA水平。在RB试验中，单次治疗48小时后检测到的热休克蛋白、CCL2和张力蛋白C的大反应减弱，表明对损伤有保护作用。卫星细胞含量和包括IGF-1在内的12个靶基因在单次治疗后30 d均升高。与对照肌相比，在那些表现出最大差异的肌肉中，ECM层粘胶蛋白- β 1和I型和III型胶原蛋白升高了6至9倍(P
The purpose of this study was to test the hypothesis that remodeling of skeletal muscle extracellular matrix (ECM) is involved in protecting human muscle against injury. Biopsies were obtained from medial gastrocnemius muscles after a single bout of electrical stimulation (B) or a repeated bout (RB) 30 d later, or 30 d after a single stimulation bout (RBc). A muscle biopsy was collected from the control leg for comparison with the stimulated leg. Satellite cell content, tenascin C, and muscle regeneration were assessed by immunohistochemistry; real-time PCR was used to measure mRNA levels of collagens, laminins, heat-shock proteins (HSPs), inflammation, and related growth factors. The large responses of HSPs, CCL2, and tenascin C detected 48 h after a single bout were attenuated in the RB trial, indicative of protection against injury. Satellite cell content and 12 target genes, including IGF-1, were elevated 30 d after a single bout. Among those displaying the greatest difference vs. control muscle, ECM laminin-beta 1 and collagen types I and III were elevated similar to 6- to 9-fold (P